ArticleMolecular and cellular biology1995
Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 83 citations in OpenAlex.
- Synergistic roles for human U1 snRNA stem-loops in pre-mRNA splicing.RNA biology · 2021Article
- Identification and Characterization of a Minimal Functional Splicing Regulatory Protein, PTBP1.Biochemistry · 2020Article
- Post-Translational Modifications in Polypyrimidine Tract Binding Proteins PTBP1 and PTBP2.Biochemistry · 2018Article
- Article
- Multiple determinants of splicing repression activity in the polypyrimidine tract binding proteins, PTBP1 and PTBP2.RNA (New York, N.Y.) · 2016Article
- Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly.Genes & development · 2014Article
- De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function.PLoS computational biology · 2014Article
- Dynamic usage of alternative splicing exons during mouse retina development.Nucleic acids research · 2011Article
- A disease-associated polymorphism alters splicing of the human CD45 phosphatase gene by disrupting combinatorial repression by heterogeneous nuclear ribonucleoproteins (hnRNPs).The Journal of biological chemistry · 2011Article
- Computational identification of tissue-specific alternative splicing elements in mouse genes from RNA-Seq.Nucleic acids research · 2010Article
- Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses.The EMBO journal · 2010Article
- Expression of 24,426 human alternative splicing events and predicted cis regulation in 48 tissues and cell lines.Nature genetics · 2008Article
- The search for alternative splicing regulators: new approaches offer a path to a splicing code.Genes & development · 2008Article
- Discovery and analysis of evolutionarily conserved intronic splicing regulatory elements.PLoS genetics · 2007Article
- A consensus CaMK IV-responsive RNA sequence mediates regulation of alternative exons in neurons.RNA (New York, N.Y.) · 2005Article
- Polypyrimidine tract binding protein blocks the 5' splice site-dependent assembly of U2AF and the prespliceosomal E complex.Molecular cell · 2005Article
- Exon repression by polypyrimidine tract binding protein.RNA (New York, N.Y.) · 2005Article
- An intronic enhancer regulates splicing of the twintron of Drosophila melanogaster prospero pre-mRNA by two different spliceosomes.Molecular and cellular biology · 2004Article
- Differentiation-induced colocalization of the KH-type splicing regulatory protein with polypyrimidine tract binding protein and the c-src pre-mRNA.Molecular biology of the cell · 2004Article
- Low tetrahydrobiopterin biosynthetic capacity of human monocytes is caused by exon skipping in 6-pyruvoyl tetrahydropterin synthase.The Biochemical journal · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The neuron-specific N1 exon of the mouse c-src transcript is normally skipped in nonneuronal cells. In this study, we examined the sequence requirements for the exclusion of this exon in nonneuronal HeLa cell nuclear extracts. We found that the repression of the N1 exon is mediated by specific intron sequences that flank the N1 exon. Mutagenesis experiments identified conserved CUCUCU elements within these intron regions that are required for the repression of N1 splicing. The addition of an RNA competitor containing the upstream regulatory sequence to the HeLa extract induced splicing of the intron downstream of N1, indicating that the competitor sequence binds to splicing repressor proteins. The similarities between this mechanism for src splicing repression and the repression of other regulated exons point to a common role of exon-spanning interactions in splicing repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.