ArticleMolecular and cellular biology1995
Rapid degradation of AU-rich element (ARE) mRNAs is activated by ribosome transit and blocked by secondary structure at any position 5' to the ARE.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 75 citations in OpenAlex.
- AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation.Nucleic acids research · 2014Article
- Cold-induced cysts of the photosynthetic dinoflagellate Lingulodinium polyedrum have an arrested circadian bioluminescence rhythm and lower levels of protein phosphorylation.Plant physiology · 2014Article
- mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end.RNA (New York, N.Y.) · 2013Article
- Association of the von Hippel-Lindau protein with AUF1 and posttranscriptional regulation of VEGFA mRNA.Molecular cancer research : MCR · 2012Article
- hnRNP Q mediates a phase-dependent translation-coupled mRNA decay of mouse Period3.Nucleic acids research · 2011Article
- Modulation of neoplastic gene regulatory pathways by the RNA-binding factor AUF1.Frontiers in bioscience (Landmark edition) · 2011Review
- Antisense targeting of thymidylate synthase (TS) mRNA increases TS gene transcription and TS protein: effects on human tumor cell sensitivity to TS enzyme-inhibiting drugs.Gene expression · 2007Article
- Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay.RNA (New York, N.Y.) · 2006Article
- Linkage analysis of the genetic determinants of T-cell IL-4 secretion, and identification of Flj20274 as a putative candidate gene.Genes and immunity · 2005Article
- AU-rich elements and associated factors: are there unifying principles?Nucleic acids research · 2005Review
- Global mRNA stabilization preferentially linked to translational repression during the endoplasmic reticulum stress response.Molecular and cellular biology · 2004Article
- Selective degradation of AU-rich mRNAs promoted by the p37 AUF1 protein isoform.Molecular and cellular biology · 2003Article
- MRNA stability and the control of gene expression: implications for human disease.Neurochemical research · 2002Review
- Ubiquitin-dependent mechanism regulates rapid turnover of AU-rich cytokine mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.Nucleic acids research · 2001Article
- The role of 3'-untranslated region (3'-UTR) mediated mRNA stability in cardiovascular pathophysiology.Molecular and cellular biochemistry · 2001Article
- Article
- Regulation of AUF1 expression via conserved alternatively spliced elements in the 3' untranslated region.Molecular and cellular biology · 1999Article
- Control of sarcoplasmic/endoplasmic-reticulum Ca2+ pump expression in cardiac and smooth muscle.The Biochemical journal · 1999Article
- Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells.Oncogene · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
The 3' noncoding region (NCR) AU-rich element (ARE) selectively confers rapid degradation on many mRNAs via a process requiring translation of the message. The role of cotranslation in destabilization of ARE mRNAs was examined by insertion of translation-blocking stable secondary structure at different sites in test mRNAs containing either the granulocyte-macrophage colony-stimulating factor (GM-CSF) ARE or a control sequence. A strong (-80 kcal/mol [1 kcal = 4.184 kJ]) but not a moderate (-30 kcal/mol) secondary structure prevented destabilization of mRNAs when inserted at any position upstream of the ARE, including in the 3' NCR. Surprisingly, a strong secondary structure did not block rapid mRNA decay when placed immediately downstream of the ARE. Studies are also presented showing that the turnover of mRNAs containing control or ARE sequences is not altered by insertion of long (1,000-nucleotide) intervening segments between the stop codon and the ARE or between the ARE and poly(A) tail. Characterization of ARE-containing mRNAs in polyadenylated and whole cytoplasmic RNA fractions failed to find evidence for decay intermediates degraded to the site of strong secondary structure from either the 5' or 3' end. From these and other data presented, this study demonstrates that complete translation of the coding region is essential for activation of rapid mRNA decay controlled by the GM-CSF ARE and that the structure of the 3' NCR can strongly influence activation. The results are consistent with activation of ARE-mediated decay by possible entry of translation-linked decay factors into the 3' NCR or translation-coupled changes in 3' NCR ribonucleoprotein structure or composition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.