ArticleMolecular and cellular biology1995
Selection of novel exon recognition elements from a pool of random sequences.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 84 citations in OpenAlex.
- Systematic characterization of short intronic splicing-regulatory elements in SMN2 pre-mRNA.Nucleic acids research · 2022Article
- Mutational bias and the protein code shape the evolution of splicing enhancers.Nature communications · 2020Article
- Context-dependent control of alternative splicing by RNA-binding proteins.Nature reviews. Genetics · 2014Review
- Intronic splicing enhancers, cognate splicing factors and context-dependent regulation rules.Nature structural & molecular biology · 2012Article
- Splicing of internal large exons is defined by novel cis-acting sequence elements.Nucleic acids research · 2012Article
- Quantitative evaluation of all hexamers as exonic splicing elements.Genome research · 2011Article
- Automatic detection of exonic splicing enhancers (ESEs) using SVMs.BMC bioinformatics · 2008Article
- Splicing regulation: from a parts list of regulatory elements to an integrated splicing code.RNA (New York, N.Y.) · 2008Review
- RNA landscape of evolution for optimal exon and intron discrimination.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Calculation of splicing potential from the Alternative Splicing Mutation Database.BMC research notes · 2008Article
- The Alternative Splicing Mutation Database: a hub for investigations of alternative splicing using mutational evidence.BMC research notes · 2008Article
- Interplay between exonic splicing enhancers, mRNA processing, and mRNA surveillance in the dystrophic Mdx mouse.PloS one · 2007Article
- Identification of a splicing enhancer in MLH1 using COMPARE, a new assay for determination of relative RNA splicing efficiencies.Human molecular genetics · 2006Article
- A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones.Genome biology · 2006Article
- Distribution of SR protein exonic splicing enhancer motifs in human protein-coding genes.Nucleic acids research · 2005Article
- Article
- RESCUE-ESE identifies candidate exonic splicing enhancers in vertebrate exons.Nucleic acids research · 2004Article
- Computational definition of sequence motifs governing constitutive exon splicing.Genes & development · 2004Article
- Sequence information for the splicing of human pre-mRNA identified by support vector machine classification.Genome research · 2003Article
- Finding signals that regulate alternative splicing in the post-genomic era.Genome biology · 2002Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A 20-nucleotide sequence close to the 3' end of the internal exon of a model two-intron, three-exon pre-mRNA (DUP184 [Z. Dominski and R. Kole, J. Biol. Chem. 269:23590-23596, 1994]) was replaced by a random 20-mer, resulting in a pool of pre-mRNAs which, like the initial DUP184 construct, were spliced in vitro by a pathway leading to predominant skipping of the internal exon. The randomized pre-mRNAs were subjected to a selection protocol, resulting in a pool enriched in pre-mRNAs that efficiently included the internal exon. Isolation and sequencing of a number of clones corresponding to the selected pre-mRNAs showed that two classes of sequences were selected from the initial pool. Most abundant among these were sequences with purine tracts similar to those in the recently identified exon-splicing enhancers while a smaller class included sequences lacking discernible purine tracts within the 20-nucleotide region. Splicing of selected pre-mRNAs showed that the purine tracts vary in their ability to promote exon inclusion and, more important, that sequences lacking purine tracts stimulate inclusion of the internal exon as efficiently as their purine-rich counterparts. The latter result indicates the existence of a novel class of exon recognition sequences or splicing enhancers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.