ArticleMolecular and cellular biology1995
Genetic enhancement of RNA-processing defects by a dominant mutation in B52, the Drosophila gene for an SR protein splicing factor.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 41 citations in OpenAlex.
- Alternative splicing perspective to prey preference of environmentally friendly biological agent Cryptolaemus montrouzieri.BMC genomics · 2024Article
- Alternative splicing and trans-splicing events revealed by analysis of the Bombyx mori transcriptome.RNA (New York, N.Y.) · 2012Article
- Negative feedback regulation among SR splicing factors encoded by Rbp1 and Rbp1-like in Drosophila.The EMBO journal · 2005Article
- A novel role for shuttling SR proteins in mRNA translation.Genes & development · 2004Article
- Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe.Molecular and cellular biology · 2000Article
- Functional characterization of SR and SR-related genes in Caenorhabditis elegans.The EMBO journal · 2000Article
- The allele-specific suppressor sup-39 alters use of cryptic splice sites in Caenorhabditis elegans.Genetics · 2000Article
- Pre-mRNA splicing by the essential Drosophila protein B52: tissue and target specificity.Molecular and cellular biology · 2000Article
- The protein kinase Clk/Sty directly modulates SR protein activity: both hyper- and hypophosphorylation inhibit splicing.Molecular and cellular biology · 1999Article
- Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster.Genetics · 1999Article
- Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements.Molecular and cellular biology · 1999Article
- Genetic analysis of the SR protein ASF/SF2: interchangeability of RS domains and negative control of splicing.Genes & development · 1998Article
- Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.Genes & development · 1998Article
- Multiple developmental requirements of noisette, the Drosophila homolog of the U2 snRNP-associated polypeptide SP3a60.Molecular and cellular biology · 1998Article
- Regulation of sex-specific selection of fruitless 5' splice sites by transformer and transformer-2.Molecular and cellular biology · 1998Article
- smg mutants affect the expression of alternatively spliced SR protein mRNAs in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- A specific RNA hairpin loop structure binds the RNA recognition motifs of the Drosophila SR protein B52.Molecular and cellular biology · 1997Article
- RNA splicing specificity determined by the coordinated action of RNA recognition motifs in SR proteins.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Functional properties of p54, a novel SR protein active in constitutive and alternative splicing.Molecular and cellular biology · 1996Article
- Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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2 authors at 2 institutions in 1 country.
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Abstract
SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-site enhancer of white-apricot (wa), a retrotransposon insertion in the second intron of the eye pigmentation gene white with a complex RNA-processing defect. B52ED also exaggerates the mutant phenotype of a distinct white allele carrying a 5' splice site mutation (wDR18), and alters the pattern of sex-specific splicing at doublesex under sensitized conditions, so that the male-specific splice is favored. In addition to being a dominant enhancer of these RNA-processing defects, B52ED is a recessive lethal allele that fails to complement other lethal alleles of B52. Comparison of B52ED with the B52+ allele from which it was derived revealed a single change in a conserved amino acid in the beta 4 strand of the first RNA-binding domain of B52, which suggests that altered RNA binding is responsible for the dominant phenotype. Reversion of the B52ED dominant allele with X rays led to the isolation of a B52 null allele. Together, these results indicate a critical role for the SR protein B52 in pre-mRNA splicing in vivo.
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