ArticleMolecular and cellular biology1995
The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed, 209 citations in OpenAlex.
- Disruption of SAP1 confers vanillin tolerance in Saccharomyces cerevisiae.Folia microbiologica · 2026Article
- Anhydrobiosis as a Model of Aging and Longevity: The Role of Autophagy and Metabolism in Yeast Cells.Microbial ecology · 2026Review
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- Next-generation stress-inducible Komagataella phaffii promoter variants.Microbial cell factories · 2025Article
- Proteomic Changes inJournal of proteome research · 2025Article
- The phenomenon of anhydrobiosis-structural and functional changes in yeast cells.Applied microbiology and biotechnology · 2025Review
- Genome-Wide Identification and Analysis of MaizePlants (Basel, Switzerland) · 2024Article
- CoVar: A generalizable machine learning approach to identify the coordinated regulators driving variational gene expression.PLoS computational biology · 2024Article
- Stress-induced inhibition of mRNA export triggers RNase III-mediated decay of theRNA (New York, N.Y.) · 2021Article
- Review
- Hog1-induced transcription of RTC3 and HSP12 is robust and occurs in cells lacking Msn2, Msn4, Hot1 and Sko1.PloS one · 2020Article
- Article
- Triterpenoid modulates the salt tolerance of lanosterol synthase deficientSaudi journal of biological sciences · 2018Article
- Heterologous Expression of the Carrot Hsp17.7 gene Increased Growth, Cell Viability, and Protein Solubility in Transformed Yeast (Saccharomyces cerevisiae) under Heat, Cold, Acid, and Osmotic Stress Conditions.Current microbiology · 2017Article
- Opposing PKA and Hog1 signals control the post-transcriptional response to glucose availability in Cryptococcus neoformans.Molecular microbiology · 2016Article
- Comparative transcriptome assembly and genome-guided profiling for Brettanomyces bruxellensis LAMAP2480 during p-coumaric acid stress.Scientific reports · 2016Article
- Respiratory metabolism and calorie restriction relieve persistent endoplasmic reticulum stress induced by calcium shortage in yeast.Scientific reports · 2016Article
- The Functional Role of eL19 and eB12 Intersubunit Bridge in the Eukaryotic Ribosome.Journal of molecular biology · 2016Article
- Involvement of glycolysis/gluconeogenesis and signaling regulatory pathways in Saccharomyces cerevisiae biofilms during fermentation.Frontiers in microbiology · 2015Article
- Histone hypoacetylation-activated genes are repressed by acetyl-CoA- and chromatin-mediated mechanism.Biochimica et biophysica acta · 2014Article
5 more citing papers are in PubMed but not listed here.
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5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The HSP12 gene encodes one of the two major small heat shock proteins of Saccharomyces cerevisiae. Hsp12 accumulates massively in yeast cells exposed to heat shock, osmostress, oxidative stress, and high concentrations of alcohol as well as in early-stationary-phase cells. We have cloned an extended 5'-flanking region of the HSP12 gene in order to identify cis-acting elements involved in regulation of this highly expressed stress gene. A detailed analysis of the HSP12 promoter region revealed that five repeats of the stress-responsive CCCCT motif (stress-responsive element [STRE]) are essential to confer wild-type induced levels on a reporter gene upon osmostress, heat shock, and entry into stationary phase. Disruption of the HOG1 and PBS2 genes leads to a dramatic decrease of the HSP12 inducibility in osmostressed cells, whereas overproduction of Hog1 produces a fivefold increase in wild-type induced levels upon a shift to a high salt concentration. On the other hand, mutations resulting in high protein kinase A (PKA) activity reduce or abolish the accumulation of the HSP12 mRNA in stressed cells. Conversely, mutants containing defective PKA catalytic subunits exhibit high basal levels of HSP12 mRNA. Taken together, these results suggest that HSP12 is a target of the high-osmolarity glycerol (HOG) response pathway under negative control of the Ras-PKA pathway. Furthermore, they confirm earlier observations that STRE-like sequences are responsive to a broad range of stresses and that the HOG and Ras-PKA pathways have antagonistic effects upon CCCCT-driven transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.