ArticleMolecular and cellular biology1995
Targeted disruption of the NIT8 gene in Chlamydomonas reinhardtii.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 74 citations in OpenAlex.
- A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis.Plant communications · 2023Article
- Channelrhodopsin-Dependent Photo-Behavioral Responses in the Unicellular Green Alga Chlamydomonas reinhardtii.Advances in experimental medicine and biology · 2021Review
- Cell Synchronization Enhances Nuclear Transformation and Genome EditingACS synthetic biology · 2020Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgaeBiotechnology for biofuels · 2019Article
- Biolistic Transformation ofFrontiers in microbiology · 2019Article
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Chlamydomonas as a model for biofuels and bio-products production.The Plant journal : for cell and molecular biology · 2015Review
- Characterization of DNA repair deficient strains of Chlamydomonas reinhardtii generated by insertional mutagenesis.PloS one · 2014Article
- High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The green algal eyespot apparatus: a primordial visual system and more?Current genetics · 2009Review
- RNA silencing in Chlamydomonas: mechanisms and tools.Current genetics · 2006Review
- Nuclear-gene targeting by using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii.Eukaryotic cell · 2005Article
- Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation.Photosynthesis research · 2005Article
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes delta-tubulin, a new member of the tubulin superfamily.Molecular biology of the cell · 1998Article
- Gene replacement by homologous recombination in the multicellular green alga Volvox carteri.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have used homologous recombination to disrupt the nuclear gene NIT8 in Chlamydomonas reinhardtii. This is the first report of targeted gene disruption of an endogenous locus in C. reinhardtii and only the second for a photosynthetic eukaryote. NIT8 encodes a protein necessary for nitrate and nitrite assimilation by C. reinhardtii. A disruption vector was constructed by placing the CRY1-1 selectable marker gene, which confers emetine resistance, within the NIT8 coding region. nit8 mutants are unable to grow on nitrate as their sole nitrogen source (Nit-) and are resistant to killing by chlorate. One of 2,000 transformants obtained after selection on emetine-chlorate medium contained a homologous insertion of five copies of the disruption plasmid into the NIT8 gene, producing an emetine-resistant, chlorate-resistant Nit- phenotype. The mutant phenotype was rescued by the wild-type NIT8 gene upon transformation. Seven other mutations at the nit8 locus, presumably resulting from homologous recombination with the disruption plasmid, were identified but were shown to be accompanied by deletions of the surrounding genomic region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.