ArticleMolecular and cellular biology1995
A central role for a single c-Myb binding site in a thymic locus control region.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Transcriptional activation of the miR-17-92 cluster is involved in the growth-promoting effects of MYB in human Ph-positive leukemia cells.Haematologica · 2019Article
- Activation of miR200 by c-Myb depends on ZEB1 expression and miR200 promoter methylation.Cell cycle (Georgetown, Tex.) · 2013Article
- Expression of Slug is regulated by c-Myb and is required for invasion and bone marrow homing of cancer cells of different origin.The Journal of biological chemistry · 2010Article
- FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci.Oncogene · 2008Article
- LymphTF-DB: a database of transcription factors involved in lymphocyte development.Genes and immunity · 2007Article
- v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter.Molecular and cellular biology · 2005Article
- Article
- The human neuroendocrine thyrotropin-releasing hormone receptor promoter is activated by the haematopoietic transcription factor c-Myb.The Biochemical journal · 2003Article
- Article
- The upstream enhancer is necessary and sufficient for the expression of the pre-T cell receptor alpha gene in immature T lymphocytes.The Journal of experimental medicine · 2001Article
- Negative regulation of CD4 gene expression by a HES-1-c-Myb complex.Molecular and cellular biology · 2001Article
- c-Myb is essential for early T cell development.Genes & development · 1999Article
- Diverse developing mouse lineages exhibit high-level c-Myb expression in immature cells and loss of expression upon differentiation.Oncogene · 1999Article
- Overexpression of an alternatively spliced form of c-Myb results in increases in transactivation and transforms avian myelomonoblasts.Journal of virology · 1998Article
- Transcriptional regulation in endoderm development: characterization of an enhancer controlling Hnf3g expression by transgenesis and targeted mutagenesis.The EMBO journal · 1997Article
- An enhancer LEF-1/TCF-1 site is essential for insertion site-independent transgene expression in thymus.Nucleic acids research · 1996Article
- Winner of the Theodore E. Woodward Award: c-Myb and the coordinate regulation of thymic genes.Transactions of the American Clinical and Climatological Association · 1996Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Locus control regions (LCRs) are powerful assemblies of cis elements that organize the actions of cell-type-specific trans-acting factors. A 2.3-kb LCR in the human adenosine deaminase (ADA) gene first intron, which controls expression in thymocytes, is composed of a 200-bp enhancer domain and extended flanking sequences that facilitate activation from within chromatin. Prior analyses have demonstrated that the enhancer contains a 28-bp core region and local adjacent augmentative cis elements. We now show that the core contains a single critical c-Myb binding site. In both transiently cotransfected human cells and stable chromatin-integrated yeast cells, c-Myb strongly transactivated reporter constructs that contained polymerized core sequences. c-Myb protein was strongly evident in T lymphoblasts in which the enhancer was active and was localized within discrete nuclear structures. Fetal murine thymus exhibited a striking concordance of endogenous c-myb expression with that of mouse ADA and human ADA LCR-directed transgene expression. Point mutation of the c-Myb site within the intact 2.3-kb LCR severely attenuated enhancer activity in transfections and LCR activity in transgenic thymocytes. Within the context of a complex enhancer and LCR, c-Myb can act as an organizer of thymocyte-specific gene expression via a single binding site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.