ArticleMolecular and cellular biology1995
The intergenic region between the divergently transcribed niiA and niaD genes of Aspergillus nidulans contains multiple NirA binding sites which act bidirectionally.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 132 citations in OpenAlex.
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- Nitric Oxide Metabolism Affects Germination inJournal of fungi (Basel, Switzerland) · 2022Article
- Genome organization and evolution of a eukaryotic nicotinate co-inducible pathway.Open biology · 2021Article
- Characterization of the promoter of the nitrate transporter-encoding gene nrtA in Aspergillus nidulans.Molecular genetics and genomics : MGG · 2020Article
- Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense.Scientific reports · 2020Article
- Reticulate evolution in eukaryotes: Origin and evolution of the nitrate assimilation pathway.PLoS genetics · 2019Article
- CoIN: co-inducible nitrate expression system for secondary metabolites inFungal biology and biotechnology · 2018Article
- A eukaryotic nicotinate-inducible gene cluster: convergent evolution in fungi and bacteria.Open biology · 2017Article
- Nitrate Assimilation inFrontiers in microbiology · 2017Article
- Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals.eLife · 2015Article
- Characterization of NpgA, a 4'-phosphopantetheinyl transferase of Aspergillus nidulans, and evidence of its involvement in fungal growth and formation of conidia and cleistothecia for development.Journal of microbiology (Seoul, Korea) · 2015Article
- Germination of Aspergillus niger conidia is triggered by nitrogen compounds related to L-amino acids.Applied and environmental microbiology · 2014Article
- Heme-biosynthetic porphobilinogen deaminase protects Aspergillus nidulans from nitrosative stress.Applied and environmental microbiology · 2012Article
- Community profiling and gene expression of fungal assimilatory nitrate reductases in agricultural soil.The ISME journal · 2011Article
- The MpkB MAP kinase plays a role in post-karyogamy processes as well as in hyphal anastomosis during sexual development in Aspergillus nidulans.Journal of microbiology (Seoul, Korea) · 2011Article
- Endocytic machinery protein SlaB is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of Aspergillus nidulans.Eukaryotic cell · 2010Article
- Isolation and growth characterization of chlorate and/or bromate resistant mutants generated by spontaneous and induced foreword mutations at several gene loci in aspergillus niger.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster.Molecular genetics and genomics : MGG · 2009Article
- A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions.Nucleic acids research · 2009Article
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6 authors at 3 institutions in 3 countries.
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Abstract
The niaD and niiA genes of Aspergillus nidulans, which code, respectively, for nitrate and nitrite reductases, are divergently transcribed, and their ATGs are separated by 1,200 bp. The genes are under the control of the positively acting NirA transcription factor, which mediates nitrate induction. The DNA binding domain of NirA was expressed as a fusion protein with the glutathione S-transferase of Schistosoma japonicum. Gel shift and footprint experiments have shown that in the intergenic region there are four binding sites for the NirA transcription factor. These sites can be represented by the nonpalindromic consensus 5'CTCCGHGG3'. Making use of a bidirectional expression vector, we have analyzed the role of each of the sites in niaD and niiA expression. The sites were numbered from the niiA side. It appeared that site 1 is necessary for the inducibility of niiA only, while sites 2, 3, and to a lesser extent 4 (which is nearer to and strongly affects niaD) act bidirectionally. The results also suggest that of the 10 binding sites for the AreA protein, which mediates nitrogen metabolite repression, those which are centrally located are physiologically important. The insertion of an unrelated upstream activating sequence into the intergenic region strongly affected the expression of both genes, irrespective of the orientation in which the element was inserted.
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