ArticleMolecular and cellular biology1995
Regulation of the DNA-binding and transcriptional activities of Xenopus laevis NFI-X by a novel C-terminal domain.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- Structural basis of Nuclear Factor 1-X DNA recognition provides prototypic insight into the NFI family.Nature communications · 2025Article
- Nuclear Factor I genomic binding associates with chromatin boundaries.BMC genomics · 2013Article
- The unique transcriptional activation domain of nuclear factor-I-X3 is critical to specifically induce marker gene expression in astrocytes.The Journal of biological chemistry · 2011Article
- Recurrent fusion of MYB and NFIB transcription factor genes in carcinomas of the breast and head and neck.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Nuclear factor one transcription factors in CNS development.Molecular neurobiology · 2009Article
- nfi-I affects behavior and life-span in C. elegans but is not essential for DNA replication or survival.BMC developmental biology · 2005Article
- NF1/X represses PDGF A-chain transcription by interacting with Sp1 and antagonizing Sp1 occupancy of the promoter.The EMBO journal · 2002Article
- Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Expression, DNA-binding specificity and transcriptional regulation of nuclear factor 1 family proteins from rat.The Biochemical journal · 1999Article
- Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo.The EMBO journal · 1998Article
- A divergent role of COOH-terminal domains in Nurr1 and Nur77 transactivation.Gene expression · 1998Article
- Second-site proviral enhancer alterations in lymphomas induced by enhancer mutants of SL3-3 murine leukemia virus: negative effect of nuclear factor 1 binding site.Journal of virology · 1997Article
- A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear factor I (NFI) family consists of sequence-specific DNA-binding proteins that activate both transcription and adenovirus DNA replication. We have characterized three new members of the NFI family that belong to the Xenopus laevis NFI-X subtype and differ in their C-termini. We show that these polypeptides can activate transcription in HeLa and Drosophila Schneider line 2 cells, using an activation domain that is subdivided into adjacent variable and subtype-specific domains each having independent activation properties in chimeric proteins. Together, these two domains constitute the full NFI-X transactivation potential. In addition, we find that the X. laevis NFI-X proteins are capable of activating adenovirus DNA replication through their conserved N-terminal DNA-binding domains. Surprisingly, their in vitro DNA-binding activities are specifically inhibited by a novel repressor domain contained within the C-terminal part, while the dimerization and replication functions per se are not affected. However, inhibition of DNA-binding activity in vitro is relieved within the cell, as transcriptional activation occurs irrespective of the presence of the repressor domain. Moreover, the region comprising the repressor domain participates in transactivation. Mechanisms that may allow the relief of DNA-binding inhibition in vivo and trigger transcriptional activation are discussed.
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