ArticleMolecular and cellular biology1995
A DNA methylation site in the male-specific P450 (Cyp 2d-9) promoter and binding of the heteromeric transcription factor GABP.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 95 citations in OpenAlex.
- Characterising sex differences of autosomal DNA methylation in whole blood using the Illumina EPIC array.Clinical epigenetics · 2022Article
- Selective Modulation of a Pan-Essential Protein as a Therapeutic Strategy in Cancer.Cancer discovery · 2021Article
- STAT5 Regulation of Sex-Dependent Hepatic CpG Methylation at Distal Regulatory Elements Mapping to Sex-Biased Genes.Molecular and cellular biology · 2021Article
- Prenatal smoke exposure induces persistentEpigenetics · 2020Article
- The Mouse Microbiome Is Required for Sex-Specific Diurnal Rhythms of Gene Expression and Metabolism.Cell metabolism · 2019Article
- Establishment and analysis of a mouse model that regulates sex-related differences in liver drug metabolism.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- The fetal programming effect of prenatal smoking on Igf1r and Igf1 methylation is organ- and sex-specific.Epigenetics · 2017Article
- Differential sensitivity to methylated DNA by ETS-family transcription factors is intrinsically encoded in their DNA-binding domains.Nucleic acids research · 2016Article
- Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling.Scientific reports · 2016Article
- Repression of multiple CYP2D genes in mouse primary hepatocytes with a single siRNA construct.In vitro cellular & developmental biology. Animal · 2015Article
- Epigenetic regulation of ADME-related genes: focus on drug metabolism and transport.Drug metabolism and disposition: the biological fate of chemicals · 2013Article
- The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.Molecular and cellular biology · 2012Article
- Gene deregulation and chronic activation in natural killer cells deficient in the transcription factor ETS1.Immunity · 2012Article
- Genomic and biochemical insights into the specificity of ETS transcription factors.Annual review of biochemistry · 2011Review
- DNA specificity determinants associate with distinct transcription factor functions.PLoS genetics · 2009Article
- Sumoylated PPARalpha mediates sex-specific gene repression and protects the liver from estrogen-induced toxicity in mice.The Journal of clinical investigation · 2009Article
- Methylation-sensitive regulation of TMS1/ASC by the Ets factor, GA-binding protein-alpha.The Journal of biological chemistry · 2009Article
- Transcription factor binding and modified histones in human bidirectional promoters.Genome research · 2007Article
- The gene encoding the fragile X RNA-binding protein is controlled by nuclear respiratory factor 2 and the CREB family of transcription factors.Nucleic acids research · 2006Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cyp 2d-9 gene encodes the male-specific steroid 16 alpha-hydroxylase in mouse liver and shares a conserved regulatory element (-100TTCCGGGC-93) with another male-specific Slp promoter. As shown with the Slp promoter (N. Yokomori, R. Moore, and M. Negishi, Proc. Natl. Acad. Sci. USA 92:1302-1306, 1995), the male-preferential demethylation also occurs at CpG/-97 in the Cyp 2d-9 promoter. The transcription factor which specifically binds to the demethylated element has been purified. The peptide sequences reveal that the factor consists of GABP alpha and GABP beta 1 with Ets and Notch motifs, respectively. Both DNase I footprinting and gel shift assays indicate that the bacterially expressed glutathione S-transferase-GABP fusion proteins bind to the regulatory element only when CpG/-97 is demethylated. Moreover, Cyp 2d-9 promoter is trans-activated by coexpression of GABP proteins in HepG2 cells. Given the additional results that CpG/-50 of the female-specific steroid 15 alpha-hydroxylase (Cyp 2a-4) promoter is preferentially demethylated in the females, the sex-specific expressions of the P450 genes correlate very well with DNA demethylation. We also conclude that GABP is a methylation-sensitive transcription factor and is a potential transcription activator of the male-specific Cyp 2d-9 promoter.
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