ArticleMolecular and cellular biology1995
Transcription in the yeast rRNA gene locus: distribution of the active gene copies and chromatin structure of their flanking regulatory sequences.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 136 citations in OpenAlex.
- Psoralen Crosslinking-Chromatin Endogenous Cleavage Assay to Examine Histone DNA Interactions of Active and Inactive rRNA Genes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- A single fiber view of the nucleosome organization in eukaryotic chromatin.Nucleic acids research · 2024Article
- Roles of ribosomal RNA in health and disease.Frontiers in RNA research · 2023Article
- Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State inNon-coding RNA · 2021Article
- Saccharomyces cerevisiae rDNA as super-hub: the region where replication, transcription and recombination meet.Cellular and molecular life sciences : CMLS · 2020Review
- Dynamic colocalization of 2 simultaneously activeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- How do cells count multi-copy genes?: "Musical Chair" model for preserving the number of rDNA copies.Current genetics · 2019Review
- Genome Organization in and around the Nucleolus.Cells · 2019Review
- Early Ongoing Speciation ofFrontiers in microbiology · 2018Article
- Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum.Scientific reports · 2017Article
- Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array.Cell reports · 2016Article
- Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder.International journal of molecular sciences · 2016Article
- A sequence-specific interaction between the Saccharomyces cerevisiae rRNA gene repeats and a locus encoding an RNA polymerase I subunit affects ribosomal DNA stability.Molecular and cellular biology · 2015Article
- Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry.European journal of histochemistry : EJH · 2014Review
- Preferential occupancy of R2 retroelements on the B chromosomes of the grasshopper Eyprepocnemis plorans.PloS one · 2014Article
- Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes.Molecular and cellular biology · 2013Article
- Global analysis of SUMO chain function reveals multiple roles in chromatin regulation.The Journal of cell biology · 2013Article
- TDP1 is an HMG chromatin protein facilitating RNA polymerase I transcription in African trypanosomes.Nucleic acids research · 2013Article
- A population genetic model for the maintenance of R2 retrotransposons in rRNA gene loci.PLoS genetics · 2013Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In growing yeast cells, about half of the 150 tandemly repeated rRNA genes are transcriptionally active and devoid of nucleosomes. By using the intercalating drug psoralen as a tool to mark accessible sites along chromatin DNA in vivo, we found that the active rRNA gene copies are rather randomly distributed along the ribosomal rRNA gene locus. Moreover, results from the analysis of a single, tagged transcription unit in the tandem array are not consistent with the presence of a specific subset of active genes that is stably maintained throughout cell divisions. In the rRNA intergenic spacers of yeast cells, an enhancer is located at the 3' end of each transcription unit, 2 kb upstream of the next promoter. Analysis of the chromatin structure along the tandem array revealed a structural link between transcription units and adjacent, 3' flanking enhancer sequences: each transcriptionally active gene is flanked by a nonnucleosomal enhancer, whereas inactive, nucleosome-packed gene copies are followed by enhancers regularly packaged in nucleosomes. From the fact that nucleosome-free enhancers were also detected in an RNA polymerase I mutant strain, we interpret these open chromatin structures as being the result of specific protein-DNA interactions that can occur before the onset of transcription. In contrast, in this mutant strain, all of the rRNA coding sequences are packaged in nucleosomal arrays. This finding indicates that the establishment of the open chromatin conformation on the activated gene copies requires elongating RNA polymerase I molecules advancing through the template.
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