ArticleMolecular and cellular biology1995
Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 95 citations in OpenAlex.
- Heat Shock Response and Heat Shock Proteins: Current Understanding and Future Opportunities in Human Diseases.International journal of molecular sciences · 2024Review
- Is there a role for HSF1 in viral infections?FEBS open bio · 2022Review
- Case report: A non-obstructive azoospermia patient with heat shock factor-2 mutation.Medicine · 2020Article
- An expanded mouse testis transcriptome and mass spectrometry defines novel proteins.Reproduction (Cambridge, England) · 2020Article
- Regulation of heat shock transcription factors and their roles in physiology and disease.Nature reviews. Molecular cell biology · 2018Review
- Human male infertility and its genetic causes.Reproductive medicine and biology · 2017Review
- Splice variants and seasonal expression of buffalo HSF genes.Cell stress & chaperones · 2015Article
- Novel isoforms of heat shock transcription factor 1, HSF1γα and HSF1γβ, regulate chaperone protein gene transcription.The Journal of biological chemistry · 2014Article
- Genome-Wide Analysis of Heat-Sensitive Alternative Splicing in Physcomitrella patens.Plant physiology · 2014Article
- A dominant-negative mutation of HSF2 associated with idiopathic azoospermia.Human genetics · 2013Article
- Article
- Inducible hsp70 in the regulation of cancer cell survival: analysis of chaperone induction, expression and activity.Cancers · 2011Article
- Transcriptional modulation of heat-shock protein gene expression.Biochemistry research international · 2011Article
- Overexpression of Colligin 2 in Glioma Vasculature is Associated with Overexpression of Heat Shock Factor 2.Gene regulation and systems biology · 2010Article
- NeuroD6 genomic signature bridging neuronal differentiation to survival via the molecular chaperone network.Journal of neuroscience research · 2010Article
- Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse Spatial gene.BMC genomics · 2004Article
- Heat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice.Molecular and cellular biology · 2002Article
- Heat shock and arsenite induce expression of the nonclassical class I histocompatibility HLA-G gene in tumor cell lines.Cell stress & chaperones · 2000Article
- Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway.Molecular and cellular biology · 1998Article
- Expression levels of heat shock factors are not functionally coupled to the rate of expression of heat shock genes.Molecular biology reports · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock factor 2 (HSF2) functions as a transcriptional regulator of heat shock protein gene expression in mammalian cells undergoing processes of differentiation and development. Our previous studies demonstrated high regulated expression and unusual constitutive DNA-binding activity of the HSF2 protein in mouse testes, suggesting that HSF2 functions to regulate heat shock protein gene expression in spermatogenic cells. The purpose of this study was to test whether HSF2 regulation in testes is associated with alterations in the HSF2 polypeptide expressed in testes relative to other mouse tissues. Our results show that mouse cells express not one but two distinct HSF2 proteins and that the levels of these HSF2 isoforms are regulated in a tissue-dependent manner. The testes express predominantly the 71-kDa HSF2-alpha isoform, while the heart and brain express primarily the 69-kDa HSF2-beta isoform. These isoforms are generated by alternative splicing of HSF2 pre-mRNA, which results in the inclusion of an 18-amino-acid coding sequence in the HSF2-alpha mRNA that is skipped in the HSF2-beta mRNA. HSF2 alternative splicing is also developmentally regulated, as our results reveal a switch in expression from the HSF2-beta mRNA isoform to the HSF2-alpha isoform during testis postnatal developmental. Transfection analysis shows that the HSF2-alpha protein, the predominant isoform expressed in testis cells, is a more potent transcriptional activator than the HSF2-beta isoform. These results reveal a new mechanism for the control of HSF2 function in mammalian cells, in which regulated alternative splicing is used to modulate HSF2 transcriptional activity in a tissue-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.