Evidence map›Paper›PMID 7565615›Full record

ArticleMolecular & general genetics : MGG1995

A temperature-sensitive lambda cI repressor functions on a modified operator in yeast cells by masking the TATA element.

H Wedler, R Wambutt

Abstract read
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In one paragraph

Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

H WedlerAGON GmbH, Berlin, FRG.
R Wambutt
Infineon Technologies (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe the construction and analysis of derivatives of the yeast TDH3 promoter in which the TATA box element has been replaced by a portion of the phage lambda operator containing a consensus TATA site flanked by binding sites for the cI repressor. Transcription of a reporter gene under the control of such a promoter is reduced in cells that express the cI repressor protein. Deletion of the native TATA element of the TDH3 promoter reduces transcription to the same extent. The cI repressor may act by "masking" the TATA element located between the repressor binding sites. Furthermore, the use of a temperature-sensitive cI repressor allowed temperature-dependent transcription of the reporter gene.

Indexed as

DNA-Binding ProteinsOperator Regions, GeneticTATA Boxalpha-AmylasesBacteriophage lambdaBase SequenceBinding SitesDNA, RecombinantGene DeletionGene Expression Regulation, FungalGene Expression Regulation, ViralGenes, FungalGlyceraldehyde-3-Phosphate DehydrogenasesMolecular Sequence DataMutagenesis, InsertionalPromoter Regions, Geneticalpha-AmylasesDNA-Binding ProteinsDNA, RecombinantGlyceraldehyde-3-Phosphate Dehydrogenasesphage repressor proteinsRepressor ProteinsViral ProteinsViral Regulatory and Accessory Proteins

Identifiers

PMID7565615
OpenAlexW1991577092

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.