ArticleMolecular & general genetics : MGG1995
DNA supercoiling in a thermotolerant mutant of Escherichia coli.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 33 citations in OpenAlex.
- Regulation of DNA Replication Initiation by Chromosome Structure.Journal of bacteriology · 2015Review
- Gene expression profiling of intrinsic thermotolerance in Escherichia coli.Current microbiology · 2006Article
- Pressures in archaeal protein coding genes: a comparative study.Comparative and functional genomics · 2003Article
- Searching for RNA genes using base-composition statistics.Nucleic acids research · 2002Article
- Mutation in the DNA gyrase A Gene of Escherichia coli that expands the quinolone resistance-determining region.Antimicrobial agents and chemotherapy · 2001Article
- Increased sensitivity to oxidative challenges associated with topA deletion in Escherichia coli.Journal of bacteriology · 2000Article
- Gatifloxacin activity against quinolone-resistant gyrase: allele-specific enhancement of bacteriostatic and bactericidal activities by the C-8-methoxy group.Antimicrobial agents and chemotherapy · 1999Article
- DNA gyrase, topoisomerase IV, and the 4-quinolones.Microbiology and molecular biology reviews : MMBR · 1997Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A spontaneously occurring, nalidixic acid-resistant (NalR), thermotolerant (T/r) mutant of Escherichia coli was isolated. Bacteriophage P1-mediated transduction showed that NalR mapped at or near gyr A, one of the two genes encoding DNA gyrase. Expression of gyrA+ from a plasmid rendered the mutant sensitive to nalidixic acid and to high temperature, the result expected for alleles mapping in gyrA. Plasmid linking number measurements, made with DNA from cells grown at 37 degrees C or shifted to 48 degrees C, revealed that supercoiling was about 12% less negative in the T/r mutant than in the parental strain. Each strain preferentially expressed two different proteins at 48 degrees C. The genetic and supercoiling data indicate that thermotolerance can arise from an alteration in DNA gyrase that lowers supercoiling. This eubacterial study, when coupled with those of archaebacteria, suggests that DNA relaxation is a general aspect of thermotolerance.
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Registered trials
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