ArticleMolecular & general genetics : MGG1995
Yeast Srp1, a nuclear protein related to Drosophila and mouse pendulin, is required for normal migration, division, and integrity of nuclei during mitosis.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Comprehensive analysis of the expression and prognostic value of ARMCs in pancreatic adenocarcinoma.BMC cancer · 2025Article
- ARMC Subfamily: Structures, Functions, Evolutions, Interactions, and Diseases.Frontiers in molecular biosciences · 2021Review
- Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.The Journal of cell biology · 2015Article
- Karyopherin alpha 1 is a putative substrate of the RAG1 ubiquitin ligase.Molecular immunology · 2009Article
- Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila.Eukaryotic cell · 2008Article
- Importin alpha binding and nuclear localization of PARP-2 is dependent on lysine 36, which is located within a predicted classical NLS.BMC cell biology · 2008Article
- Regulation of Nuclear Import During Differentiation; The IMP alpha Gene Family and Spermatogenesis.Current genomics · 2007Article
- Article
- Transcript profiling of Wilms tumors reveals connections to kidney morphogenesis and expression patterns associated with anaplasia.Oncogene · 2005Article
- Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p.Molecular genetics and genomics : MGG · 2004Article
- Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha.The Biochemical journal · 2003Article
- A role for Caenorhabditis elegans importin IMA-2 in germ line and embryonic mitosis.Molecular biology of the cell · 2002Article
- Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation.Molecular and cellular biology · 2000Article
- Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms.The Journal of cell biology · 2000Article
- Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1.Molecular and cellular biology · 2000Article
- RanBP3 contains an unusual nuclear localization signal that is imported preferentially by importin-alpha3.Molecular and cellular biology · 1999Article
- Cse1p is involved in export of yeast importin alpha from the nucleus.Molecular and cellular biology · 1998Article
- Nuclear transport: run by Ran?American journal of human genetics · 1998Review
- Evolutionary specialization of the nuclear targeting apparatus.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper describes genes from yeast and mouse with significant sequence similarities to a Drosophila gene that encodes the blood cell tumor suppressor pendulin. The protein encoded by the yeast gene, Srp1p, and mouse pendulin share 42% and 51% amino acid identity with Drosophila pendulin, respectively. All three proteins consist of 10.5 degenerate tandem repeats of approximately 42 amino acids each. Similar repeats occur in a superfamily of proteins that includes the Drosophila Armadillo protein. All three proteins contain a consensus sequence for a bipartite nuclear localization signal (NLS) in the N-terminal domain, which is not part of the repeat structure. Confocal microscopic analysis of yeast cells stained with antibodies against Srp1p reveals that this protein is intranuclear throughout the cell cycle. Targeted gene disruption shows that SRP1 is an essential gene. Despite their sequence similarities, Drosophila and mouse pendulin are unable to rescue the lethality of an SRP1 disruption. We demonstrate that yeast cells depleted of Srp1p arrest in mitosis with a G2 content of DNA. Arrested cells display abnormal structures and orientations of the mitotic spindles, aberrant segregation of the chromatin and the nuclei, and threads of chromatin emanating from the bulk of nuclear DNA. This phenotype suggests that Srp1p is required for the normal function of microtubules and the spindle pole bodies, as well as for nuclear integrity. We suggest that Srp1p interacts with multiple components of the cell nucleus that are required for mitosis and discuss its functional similarities to, and differences from Drosophila pendulin.
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