ArticleThe Journal of membrane biology1995
A novel type of cell-cell cooperation between epithelial cells.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- The βInternational journal of molecular sciences · 2022Article
- Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity.The Journal of membrane biology · 2019Article
- The Apical Localization of NaFrontiers in physiology · 2016Article
- Ouabain modulates ciliogenesis in epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The polarized distribution of Na+,K+-ATPase: role of the interaction between {beta} subunits.Molecular biology of the cell · 2010Article
- Ouabain modulates epithelial cell tight junction.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- beta-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells.American journal of physiology. Renal physiology · 2008Article
- Contacts and cooperation between cells depend on the hormone ouabain.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells.Molecular biology of the cell · 2005Article
- Ouabain binding to Na+,K+-ATPase relaxes cell attachment and sends a specific signal (NACos) to the nucleus.The Journal of membrane biology · 2004Article
- Gap junctional communication in morphogenesis.Progress in biophysics and molecular biologyReview
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ma104 cells (renal, epithelial) have a peculiar way of resisting ouabain: their Na+,K(+)-pumps bind the drug with high affinity, cellular K+ is lost and cell division arrested, but cells do not detach as most cell types do. Then, if up to 4 days later the drug is removed, Ma104 cells recover K+ and resume proliferation (Contreras et al., 1994). In the present work, we investigate whether Ma104 cells are able to protect ouabain-sensitive MDCK cells in co-culture. The main finding is that they do, but in this case protection is not elicited by the usual mechanism of maintaining the K+ content of neighboring cells through cell-cell communications. Ma104 cells treated with ouabain simply remain attached to the substrate and to their MDCK neighbors, and both cells lose K+. This attachment includes tight junctions, because the transepithelial electrical resistance of the monolayers is not abolished by ouabain. Although the beta-subunit of the Na+,K(+)-ATPase is known to possess molecular characteristics of cell-cell attachment molecules, attachment between Ma104-MDCK cells does not seem to be mediated by this enzyme, as immunofluorescence analysis reveals that Na+,K(+)-ATPase is only inserted in the plasma membrane facing a neighboring cell of the same type.
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