ArticleThe Journal of membrane biology1995
Ouabain resistance of the epithelial cell line (Ma104) is not due to lack of affinity of its pumps for the drug.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 36 citations in OpenAlex.
- The influence of Ouabain on human dendritic cells maturation.Mediators of inflammation · 2014Article
- The death of ouabain-treated renal epithelial C11-MDCK cells is not mediated by swelling-induced plasma membrane rupture.The Journal of membrane biology · 2011Article
- Ouabain modulates epithelial cell tight junction.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- [Na+]i/[K+]i -independent death of ouabain-treated renal epithelial cells is not mediated by Na+,K+ -ATPase internalization and de novo gene expression.Pflugers Archiv : European journal of physiology · 2008Article
- Contacts and cooperation between cells depend on the hormone ouabain.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Intracellular monovalent ions as second messengers.The Journal of membrane biology · 2006Review
- Modest intracellular acidification suppresses death signaling in ouabain-treated cells.Pflugers Archiv : European journal of physiology · 2006Article
- Na+/K+ pump and endothelial cell survival: [Na+]i/[K+]i-independent necrosis triggered by ouabain, and protection against apoptosis mediated by elevation of [Na+]i.Pflugers Archiv : European journal of physiology · 2004Article
- Ouabain binding to Na+,K+-ATPase relaxes cell attachment and sends a specific signal (NACos) to the nucleus.The Journal of membrane biology · 2004Article
- A novel type of cell-cell cooperation between epithelial cells.The Journal of membrane biology · 1995Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Na+, K(+)-pumps of most eukaryotic animal cells bind ouabain with high affinity, stop pumping, and consequently loose K+, detach from each other and from the substrate, and die. Lack of affinity for the drug results in ouabain resistance. In this work, we report that Ma104 cells (epithelial from Rhesus monkey kidney) have a novel form of ouabain-resistance: they bind the drug with high affinity (Km about 4 x 10(-8) M), they loose their K+ and stop proliferating but, in spite of these, up to 100% of the cells remain attached in 1.0 microM ouabain, and 53% in 1.0 mM. When 4 days later ouabain is removed from the culture medium, cells regain K+ and resume proliferation. Strophanthidin, a drug that attaches less firmly than ouabain, produces a similar phenomenon, but allows a considerably faster recovery. This reversal may be associated to the fact that, while in ouabain-sensitive MDCK cells Na+, K(+)-ATPases blocked by the drug are retrieved from the plasma membrane, those in Ma104 cells remain at the cell-cell border, as if they were cell-cell attaching molecules. Cycloheximide (10 micrograms/ml) and chloroquine (10 microM) impair this recovery, suggesting that it also depends on the synthesis and insertion of a crucial protein component, that may be different from the pump itself. Therefore ouabain resistance of Ma104 cells is not due to a lack of affinity for the drug, but to a failure of its Na+, K(+)-ATPases to detach from the plasma membrane in spite of being blocked by ouabain.
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