ArticleThe Journal of membrane biology1995
Modulation of neuronal calcium channels by arachidonic acid and related substances.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels.Frontiers in physiology · 2017Review
- A mechanism underlying the effects of polyunsaturated fatty acids on breast cancer.International journal of molecular medicine · 2012Article
- Regulation of voltage-gated Ca2+ channels by lipids.Cell calcium · 2009Review
- Arachidonic acid and ion channels: an update.British journal of pharmacology · 2008Review
- Mechanism of arachidonic acid modulation of the T-type Ca2+ channel alpha1G.The Journal of general physiology · 2004Article
- Effects of arachidonic acid on unitary calcium currents in rat sympathetic neurons.The Journal of physiology · 2000Article
- Arachidonic acid stimulates a novel cocaine-sensitive cation conductance associated with the human dopamine transporter.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Prevention of ischemia-induced cardiac sudden death by n-3 polyunsaturated fatty acids in dogs.Lipids · 1997Article
- Evidence that free polyunsaturated fatty acids modify Na+ channels by directly binding to the channel proteins.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low-voltage-activated (l-v-a) and high-voltage-activated (h-v-a) Ca2+ currents (ICa) were recorded in whole-cell voltage clamped NG108-15 neuroblastoma x glioma hybrid cells. We studied the effects of arachidonic acid (AA), oleic acid, myristic acid and of the positively charged compounds tetradecyltrimethylammonium (C14TMA) and sphingosine. At pulse potentials > -20 mV, AA (25-100 microM) decreased l-v-a and h-v-a ICa equally. The decrease developed slowly and became continually stronger with increasing time of application. It was accompanied by a small negative shift and a slight flattening of the activation and inactivation curves of the l-v-a ICa. The shift of the activation curve manifested itself in a small increase of l-v-a ICa at pulse potentials < -30 mV. The effects were only partly reversible. The AA effect was not prevented by 50 microM 5, 8, 11, 14-eicosatetraynoic acid, an inhibitor of the AA metabolism, and not mimicked by 0.1-1 microM phorbol 12, 13-dibutyrate, an activator of protein kinase C. Probably, AA directly affects the channel protein or its lipid environment. Oleic and myristic acid acted similarly to AA but were much less effective. The positively charged compounds C14TMA and sphingosine had a different effect: They shifted the activation curve of l-v-a ICa in the positive direction and suppressed l-v-a more than h-v-a ICa; their effect reached a steady-state within 5-10 min and was readily reversible. C14TMA blocked l-v-a ICa with an IC50 of 4.2 microM while sphingosine was less potent.
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