ArticleThe Journal of clinical investigation1995
Possible links between glucose-induced changes in the energy state of pancreatic B cells and insulin release. Unmasking by decreasing a stable pool of adenine nucleotides in mouse islets.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 115 citations in OpenAlex.
- Hypertonicity during a rapid rise in D-glucose mediates first-phase insulin secretion.Frontiers in endocrinology · 2024Article
- Short-Term Inhibition of Translation by Cycloheximide Concurrently Affects Mitochondrial Function and Insulin Secretion in Islets from Female Mice.International journal of molecular sciences · 2023Article
- Secretory Conservation in Insulin Producing Cells: Is There a System-Level Law of Mass Action in Biology?ACS omega · 2023Article
- The Dynamics of Calcium Signaling in Beta Cells-A Discussion on the Comparison of Experimental and Modelling Data.International journal of molecular sciences · 2023Article
- What Is the Metabolic Amplification of Insulin Secretion and Is It (Still) Relevant?Metabolites · 2021Review
- Islet O-GlcNAcylation Is Required for Lipid Potentiation of Insulin Secretion through SERCA2.Cell reports · 2020Article
- Metabolic activation-driven mitochondrial hyperpolarization predicts insulin secretion in human pancreatic beta-cells.Biochimica et biophysica acta. Bioenergetics · 2018Article
- Insulin Resistance in Human iPS Cells Reduces Mitochondrial Size and Function.Scientific reports · 2016Article
- Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells.PloS one · 2016Article
- An extract of Artemisia dracunculus L. stimulates insulin secretion from β cells, activates AMPK and suppresses inflammation.Journal of ethnopharmacology · 2015Article
- Protein phosphatases in pancreatic islets.The Journal of endocrinology · 2014Review
- Purinergic signalling and diabetes.Purinergic signalling · 2013Review
- Adenylate kinase 2 links mitochondrial energy metabolism to the induction of the unfolded protein response.The Journal of biological chemistry · 2011Article
- A highly energetic process couples calcium influx through L-type calcium channels to insulin secretion in pancreatic beta-cells.American journal of physiology. Endocrinology and metabolism · 2009Article
- Purinergic receptors in the endocrine and exocrine pancreas.Purinergic signalling · 2008Article
- Acute nutrient regulation of the unfolded protein response and integrated stress response in cultured rat pancreatic islets.Diabetologia · 2007Article
- AMP-activated protein kinase signaling in metabolic regulation.The Journal of clinical investigation · 2006Review
- Anaplerosis via pyruvate carboxylase is required for the fuel-induced rise in the ATP:ADP ratio in rat pancreatic islets.Diabetologia · 2006Article
- Stronger control of ATP/ADP by proton leak in pancreatic beta-cells than skeletal muscle mitochondria.The Biochemical journal · 2006Article
- Kir6.2 mutations causing neonatal diabetes provide new insights into Kir6.2-SUR1 interactions.The EMBO journal · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whether adenine nucleotides in pancreatic B cells serve as second messengers during glucose stimulation of insulin secretion remains disputed. Our hypothesis was that the actual changes in ATP and ADP are obscured by the large pool of adenine nucleotides (ATP/ADP ratio close to 1) in insulin granules. Therefore, mouse islets were degranulated acutely with a cocktail of glucose, KCl, forskolin, and phorbol ester or during overnight culture in RPMI-1640 medium containing 10 mM glucose. When these islets were then incubated in 0 glucose + azide (to minimize cytoplasmic and mitochondrial adenine nucleotides), their content in ATP + ADP + AMP was decreased in proportion to the decrease in insulin stores. After incubation in 10 mM glucose (no azide), the ATP/ADP ratio increased from 2.4 to > 8 in cultured islets, and only from 2 to < 4 in fresh islets. These differences were not explained by changes in glucose oxidation. The glucose dependency (0-30 mM) of the changes in insulin secretion and in the ATP/ADP ratio were then compared in the same islets. In nondegranulated, fresh islets, the ATP/ADP ratio increased between 0 and 10 mM glucose and then stabilized although insulin release kept increasing. In degranulated islets, the ATP/ADP ratio also increased between 0 and 10 mM glucose, but a further increase still occurred between 10 and 20 mM glucose, in parallel with the stimulation of insulin release. In conclusion, decreasing the granular pool of ATP and ADP unmasks large changes in the ATP/ADP ratio and a glucose dependency which persists within the range of stimulatory concentrations. The ATP/ADP ratio might thus serve as a coupling factor between glucose metabolism and insulin release.
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