ArticleJournal of clinical microbiology1995
Quantitative PCR for human herpesviruses 6 and 7.
Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 84 citations in OpenAlex.
- Deficient EBV-specific B- and T-cell response in patients with chronic fatigue syndrome.PloS one · 2014Article
- Calibrated real-time PCR assay for quantitation of human herpesvirus 8 DNA in biological fluids.Journal of clinical microbiology · 2002Article
- Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva.Journal of clinical microbiology · 2002Article
- Gene expression profile of herpesvirus-infected T cells obtained using immunomicroarrays: induction of proinflammatory mechanisms.Journal of virology · 2001Article
- Real-time quantitative PCR for human herpesvirus 6 DNA.Journal of clinical microbiology · 2000Article
- An immunoblot assay for detection of immunoglobulin M antibody to human herpesvirus 6.Clinical and diagnostic laboratory immunology · 2000Article
- Application of competitive PCR to cerebrospinal fluid samples from patients with herpes simplex encephalitis.Journal of clinical microbiology · 1998Article
- Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays.Journal of clinical microbiology · 1998Article
- Detection of human herpesvirus 6 DNA in serum by a microplate PCR-hybridization assay.Journal of clinical microbiology · 1998Article
- Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR.Journal of clinical microbiology · 1997Article
- Human herpesvirus 6.Clinical microbiology reviews · 1997Review
- Role of the extracellular domain of human herpesvirus 7 glycoprotein B in virus binding to cell surface heparan sulfate proteoglycans.Journal of virology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative PCR assay for the detection of human herpesvirus 6 (HHV-6) (variants A and B) and HHV-7 DNAs in clinical samples was developed. The assay uses a nonhomologous internal standard (IS) for each virus that is coamplified with the wild-type target sequence in the same vial and with the same pair of primers. This method allows for a correction of the variability of efficiency of the PCR technique. A standard curve is constructed for each experiment by coamplification of known quantities of the cloned HHV-6 or HHV-7 target templates with the respective IS. Absolute quantitation of the test samples is then achieved by determining the viral target/IS ratio of the hybridization signals of the amplification products and plotting this value against the standard curve. Using this assay, we quantitated the amount of HHV-6 or HHV-7 DNA in infected cell cultures and demonstrated an inhibitory effect of phosphonoformic acid on the replication of HHV-6 and HHV-7 in vitro. As the first clinical application of this procedure, we performed preliminary measurements of the loads of HHV-6 and HHV-7 in lymph nodes from patients with Hodgkin's disease and AIDS. Application of this quantitative PCR method should be helpful for elucidating the pathogenic roles of HHV-6 and HHV-7.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.