ArticleThe Journal of cell biology1995
gamma-tubulin is a minus end-specific microtubule binding protein.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 95 citations in OpenAlex.
- Dynamic Changes of Brain Cilia Transcriptomes across the Human Lifespan.International journal of molecular sciences · 2021Article
- FtsZ filament capping by MciZ, a developmental regulator of bacterial division.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Molecular insight into γ-γ tubulin lateral interactions within the γ-tubulin ring complex (γ-TuRC).Journal of computer-aided molecular design · 2014Article
- Intracellular transport of the measles virus ribonucleoprotein complex is mediated by Rab11A-positive recycling endosomes and drives virus release from the apical membrane of polarized epithelial cells.Journal of virology · 2013Article
- Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion.PloS one · 2013Article
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- The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus.Molecular biology of the cell · 2006Article
- Lupinus albus gamma-tubulin: mRNA and protein accumulation during development and in response to darkness.Planta · 2004Article
- Characterization of a new gammaTuRC subunit with WD repeats.Molecular biology of the cell · 2003Article
- Association of brain gamma-tubulins with alpha beta-tubulin dimers.The Biochemical journal · 2002Article
- Reconstitution and characterization of budding yeast gamma-tubulin complex.Molecular biology of the cell · 2002Article
- Conditional mutations in gamma-tubulin reveal its involvement in chromosome segregation and cytokinesis.Molecular biology of the cell · 2001Article
- Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos.Molecular biology of the cell · 2001Article
- Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits.The Journal of cell biology · 2000Article
- A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p.Molecular biology of the cell · 2000Article
- The C-terminus of tubulin increases cytoplasmic dynein and kinesin processivity.Biophysical journal · 2000Article
- Human 76p: A new member of the gamma-tubulin-associated protein family.The Journal of cell biology · 1999Article
- GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein.The Journal of cell biology · 1999Article
- Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation.The EMBO journal · 1998Article
- ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of microtubules in mediating chromosome segregation during mitosis is well-recognized. In addition, interphase cells depend upon a radial and uniform orientation of microtubules, which are intrinsically asymmetric polymers, for the directional transport of many cytoplasmic components and for the maintenance of the structural integrity of certain organelles. The slow growing minus ends of microtubules are linked to the centrosome ensuring extension of the fast growing plus ends toward the cell periphery. However, the molecular mechanism of this linkage is not clear. One hypothesis is that gamma-tubulin, located at the centrosome, binds to the minus ends of microtubules. To test this model, we synthesized radiolabeled gamma-tubulin in vitro. We demonstrate here biochemically a specific, saturable, and tight (Kd = 10(-10) M) interaction of gamma-tubulin and microtubule ends with a stoichiometry of 12.6 +/- 4.9 molecules of gamma-tubulin per microtubule. In addition, we designed an in vitro assay to visualize gamma-tubulin at the minus ends of axonemal microtubules. These data show that gamma-tubulin represents the first protein to bind microtubule minus ends and might be responsible for mediating the link between microtubules and the centrosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.