ArticleThe Journal of cell biology1995
Isolation and characterization of nuclear envelopes from the yeast Saccharomyces.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 69 citations in OpenAlex.
- A general method for quantitative fractionation of mammalian cells.The Journal of cell biology · 2023Article
- Evolution and diversification of the nuclear envelope.Nucleus (Austin, Tex.) · 2021Article
- The Many Faces of Lipids in Genome Stability (and How to Unmask Them).International journal of molecular sciences · 2021Review
- Dissecting the Structural Dynamics of the Nuclear Pore Complex.Molecular cell · 2021Article
- Joint inference and alignment of genome structures enables characterization of compartment-independent reorganization across cell types.Epigenetics & chromatin · 2019Article
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- Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression.Nucleic acids research · 2016Article
- Analysis of membrane proteins localizing to the inner nuclear envelope in living cells.The Journal of cell biology · 2016Article
- Motor-driven motility of fungal nuclear pores organizes chromosomes and fosters nucleocytoplasmic transport.The Journal of cell biology · 2012Article
- Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution.The Journal of cell biology · 2010Article
- Proteomics of Saccharomyces cerevisiae Organelles.Molecular & cellular proteomics : MCP · 2010Review
- The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly.The Journal of cell biology · 2009Article
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- The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.The Journal of cell biology · 2007Article
- The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo.Molecular biology of the cell · 2007Article
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- Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein.The Biochemical journal · 2005Article
- Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The Ran GTPase cycle is required for yeast nuclear pore complex assembly.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a large scale enrichment procedure to prepare yeast nuclear envelopes (NEs). These NEs can be stripped of peripheral proteins to produce a heparin-extracted NE (H-NE) fraction highly enriched in integral membrane proteins. Extraction of H-NEs with detergents revealed previously uncharacterized ring structures associated with the NE that apparently stabilize the grommets of the nuclear pore complexes (NPCs). The high yields obtained throughout the fractionation procedure allowed balance-sheet tabulation of the subcellular distribution of various NE and non-NE proteins. Thus we found that 20% of endoplasmic reticulum (ER) marker proteins are localized at the NE. Using a novel monospecific mAb made against proteins in the H-NE fraction and found to be directed against the pore membrane protein POM152, we showed that while the majority of POM152 is localized in the NE at the NPC, a proportion of this protein is also present in the ER. This ER pool of POM152 is likely to be involved in the duplication of nuclear pores and NPCs during S-phase. Both the NEs and H-NEs were found to be competent for the in vitro posttranslational translocation of prepro-alpha-factor. They may also be suitable to investigate other ER- and NE-associated functions in cell-free systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.