ArticleThe Journal of cell biology1995
Expression of the MRP gene-encoded conjugate export pump in liver and its selective absence from the canalicular membrane in transport-deficient mutant hepatocytes.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 228 citations in OpenAlex.
- MRP2 and the Transport Kinetics of Cysteine Conjugates of Inorganic Mercury.Biological trace element research · 2018Article
- Boosted coupling of ATP hydrolysis to substrate transport upon cooperative estradiol-17-β-D-glucuronide binding in a Drosophila ATP binding cassette type-C transporter.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Expression of Tight Junction Components in Hepatocyte-Like Cells Differentiated from Human Embryonic Stem Cells.Pathology oncology research : POR · 2015Article
- Novel Hg2+-induced nephropathy in rats and mice lacking Mrp2: evidence of axial heterogeneity in the handling of Hg2+ along the proximal tubule.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- ABC transporters in fish species: a review.Frontiers in physiology · 2014Review
- Bile formation and secretion.Comprehensive Physiology · 2013Review
- Expression of multidrug resistance-associated protein 2 in human gallbladder carcinoma.BioMed research international · 2013Article
- Placental and fetal disposition of mercuric ions in rats exposed to methylmercury: role of Mrp2.Reproductive toxicology (Elmsford, N.Y.) · 2012Article
- A perspective on efflux transport proteins in the liver.Clinical pharmacology and therapeutics · 2012Review
- Evaluation of (99) (m)TcN-MPO as a new myocardial perfusion imaging agent in normal dogs and in an acute myocardial infarction canine model: comparison with (99) (m)Tc-sestamibi.Molecular imaging and biology · 2011Article
- Seventy-five percent nephrectomy and the disposition of inorganic mercury in 2,3-dimercaptopropanesulfonic acid-treated rats lacking functional multidrug-resistance protein 2.The Journal of pharmacology and experimental therapeutics · 2010Article
- Minimizing liver uptake of cationic Tc radiotracers with ether and crown ether functional groups.World journal of hepatology · 2010Article
- ABC transporters and isothiocyanates: potential for pharmacokinetic diet-drug interactions.Biopharmaceutics & drug disposition · 2009Article
- Role of multidrug transporters in neurotherapeutics.Annals of Indian Academy of Neurology · 2009Article
- Evaluation of (64)Cu(DO3A-xy-TPEP) as a potential PET radiotracer for monitoring tumor multidrug resistance.Bioconjugate chemistry · 2009Article
- MRP2 and the DMPS- and DMSA-mediated elimination of mercury in TR(-) and control rats exposed to thiol S-conjugates of inorganic mercury.Toxicological sciences : an official journal of the Society of Toxicology · 2008Article
- Multidrug resistance proteins and the renal elimination of inorganic mercury mediated by 2,3-dimercaptopropane-1-sulfonic acid and meso-2,3-dimercaptosuccinic acid.The Journal of pharmacology and experimental therapeutics · 2008Article
- Exploitation of bile acid transport systems in prodrug design.Molecules (Basel, Switzerland) · 2007Review
- The apical conjugate efflux pump ABCC2 (MRP2).Pflugers Archiv : European journal of physiology · 2007Review
- Involvement of the drug transporters p glycoprotein and multidrug resistance-associated protein Mrp2 in telithromycin transport.Antimicrobial agents and chemotherapy · 2006Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that the multi-drug resistance protein (MRP) mediates the ATP-dependent membrane transport of glutathione S-conjugates and additional amphiphilic organic anions. In the present study we demonstrate the expression of MRP in hepatocytes where it functions in hepatobiliary excretion. Analysis by reverse transcription-PCR of human and normal rat liver mRNA resulted in two expected cDNA fragments of MRP. Four different antibodies against MRP reacted on immunoblots with the glycoprotein of about 190 kD from human canalicular as well as basolateral hepatocyte membrane preparations. A polyclonal antibody directed against the carboxy-terminal sequence of MRP detected the rat homolog of MRP in liver. Double immunofluorescence microscopy and confocal laser scanning microscopy showed the presence of human MRP and rat Mrp in the canalicular as well as in the lateral membrane domains of hepatocytes. The transport function of the mrp gene-encoded conjugate export pump was assayed in plasma membrane vesicles with leukotriene C4 as a high-affinity glutathione S-conjugate substrate. The deficient ATP-dependent conjugate transport in canalicular membranes from TR- mutant rat hepatocytes was associated with a lack of amplification of one of the mrp cDNA fragments and with a selective loss of Mrp on immunoblots of canalicular membranes. Double immunofluorescence microscopy of livers from transport-deficient TR- mutant rats localized Mrp only to the lateral but not to the canalicular membrane. Our results indicate that the absence of Mrp or an isoform of Mrp from the canalicular membrane is the basis for the hereditary defect of the hepatobiliary excretion of anionic conjugates by the transport-deficient hepatocyte.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.