ArticleThe Journal of cell biology1995
Virus-mediated release of endosomal content in vitro: different behavior of adenovirus and rhinovirus serotype 2.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 136 citations in OpenAlex.
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- Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
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- "Repair Me if You Can": Membrane Damage, Response, and Control from the Viral Perspective.Cells · 2020Review
- How non-enveloped viruses hijack host machineries to cause infection.Advances in virus research · 2019Article
- Cleavage of the C-Terminal Fragment of Reovirus μ1 Is Required for Optimal Infectivity.Journal of virology · 2018Article
- Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Surveillance for Intracellular Antibody by Cytosolic Fc Receptor TRIM21.Antibodies (Basel, Switzerland) · 2016Review
- Principles of Virus Uncoating: Cues and the Snooker Ball.Traffic (Copenhagen, Denmark) · 2016Review
- Viral entry pathways: the example of common cold viruses.Wiener medizinische Wochenschrift (1946) · 2016Review
- Journey to the Center of the Cell: Current Nanocarrier Design Strategies Targeting Biopharmaceuticals to the Cytoplasm and Nucleus.Current pharmaceutical design · 2016Review
- Cell Walls and the Convergent Evolution of the Viral Envelope.Microbiology and molecular biology reviews : MMBR · 2015Review
- Review
- The role of cellular adhesion molecules in virus attachment and entry.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- The VP4 peptide of hepatitis A virus ruptures membranes through formation of discrete pores.Journal of virology · 2014Article
- Coat as a dagger: the use of capsid proteins to perforate membranes during non-enveloped DNA viruses trafficking.Viruses · 2014Review
- Basics and recent advances in peptide and protein drug delivery.Therapeutic delivery · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endosomal penetration by nonenveloped viruses might be accomplished by either local breakdown of the endosomal membrane (e.g., adenovirus) or formation of a membrane-spanning pore by capsid proteins. Uncoating of the nonenveloped virus human rhinovirus serotype 2 (HRV2) has been shown to occur from late endosomes and to be entirely dependent on the acidic pH in this compartment (Prchla, E., E. Kuechler, D. Blaas, and R. Fuchs. 1994. J. Virol. 68: 3713-3723). To investigate further the mechanism of uncoating of HRV2, an in vitro assay was established to test viruses or virus-derived peptides for their capacity to release cointernalized biotin-dextran of different molecular mass (10 and 70 kD) from isolated endosomes. The suitability of the assay was demonstrated by use of a fusogenic peptide derived from influenza virus hemagglutinin (GALA-INF3). Whereas adenovirus induced a low pH-dependent release of up to 46% of the internalized biotin-dextran and did not show any significant size selectivity (as expected for endosome disruption), HRV2 mediated release of 27% of the 10 kD dextran and only traces of the 70-kD dextran. Similarly, GALA-INF3-induced release of biotin-dextran was also size dependent. The potential role of the capsid protein VP1 in HRV2 uncoating in vivo was also substantiated in our in vitro system using an amphipathic, NH2-terminal peptide of VP1. Taken together, these data favor the model of a specific pore-forming mechanism for HRV2 uncoating which is in contrast to the membrane-disrupting mechanism of adenovirus.
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