ArticleThe Journal of cell biology1995
Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 109 citations in OpenAlex.
- Uptake of ricinB-quantum dot nanoparticles by a macropinocytosis-like mechanism.Journal of nanobiotechnology · 2012Article
- Increased availability of angiotensin AT 1 receptors leads to sustained arterial constriction to angiotensin II in diabetes - role for Rho-kinase activation.British journal of pharmacology · 2011Article
- Xenopus Kazrin interacts with ARVCF-catenin, spectrin and p190B RhoGAP, and modulates RhoA activity and epithelial integrity.Journal of cell science · 2010Article
- Constitutive recycling of the store-operated Ca2+ channel Orai1 and its internalization during meiosis.The Journal of cell biology · 2010Article
- Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation.Developmental biology · 2008Article
- Lack of evidence for direct phosphorylation of recombinantly expressed P2X(2) and P2X (3) receptors by protein kinase C.Purinergic signalling · 2007Article
- Dual control of cardiac Na+ Ca2+ exchange by PIP(2): analysis of the surface membrane fraction by extracellular cysteine PEGylation.The Journal of physiology · 2007Article
- Rho regulates membrane transport in the endocytic pathway to control plasma membrane specialization in oligodendroglial cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Rho1 regulates signaling events required for proper Drosophila embryonic development.Developmental biology · 2005Article
- Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway.Molecular biology of the cell · 2003Article
- Inhibition of Rho GTPases with protein prenyltransferase inhibitors prevents leukocyte recruitment to the central nervous system and attenuates clinical signs of disease in an animal model of multiple sclerosis.Journal of immunology (Baltimore, Md. : 1950) · 2002Article
- An improved method for real-time monitoring of membrane capacitance in Xenopus laevis oocytes.Biophysical journal · 2002Article
- RAC1 regulates adherens junctions through endocytosis of E-cadherin.Molecular biology of the cell · 2001Article
- Phosphoinositide-3 kinase binds to a proline-rich motif in the Na+, K+-ATPase alpha subunit and regulates its trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Syndapin isoforms participate in receptor-mediated endocytosis and actin organization.The Journal of cell biology · 2000Article
- Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.Molecular biology of the cell · 2000Article
- Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA.Molecular biology of the cell · 1999Article
- Rab5 induces Rac-independent lamellipodia formation and cell migration.Molecular biology of the cell · 1999Article
- Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes.The Journal of physiology · 1999Article
- RacF1, a novel member of the Rho protein family in Dictyostelium discoideum, associates transiently with cell contact areas, macropinosomes, and phagosomes.Molecular biology of the cell · 1999Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
To study an endocytotic role of the GTP-binding protein RhoA in Xenopus oocytes, we have monitored changes in the surface expression of sodium pumps, the surface area of the oocyte and the uptake of the fluid-phase marker inulin. Xenopus oocytes possess intracellular sodium pumps that are continuously exchanged for surface sodium pumps by constitutive endo- and exocytosis. Injection of Clostridium botulinum C3 exoenzyme, which inactivates Rho by ADP-ribosylation, induced a redistribution of virtually all intracellular sodium pumps to the plasma membrane and increased the surface area of the oocytes. The identical effects were caused by injection of ADP-ribosylated recombinant RhoA into oocytes. The C3 exoenzyme acts by blocking constitutive endocytosis in oocytes, as determined using a mAb to the beta 1 subunit of the mouse sodium pump as a reporter molecule and oocytes expressing heterologous sodium pumps. In contrast, an increase in endocytosis and a decrease in the surface area was induced by injection of recombinant Val14-RhoA protein or Val14-rhoA cRNA. PMA stimulated sodium pump endocytosis, an effect that was blocked by a specific inhibitor of protein kinase C (Gö 16) or by ADP-ribosylation of Rho by C3. Similarly, the phorbol ester-induced increase in fluid-phase endocytosis in oocytes was inhibited by Gö 16, C3 transferase, or by injection of ADP-ribosylated RhoA. In contrast to C3 transferase, C. botulinum C2 transferase, which ADP-ribosylates actin, had no effect on sodium pump endocytosis or PMA-stimulated fluid-phase endocytosis. The data suggests that RhoA is an essential component of a presumably clathrin-independent endocytic pathway in Xenopus oocytes which can be regulated by protein kinase C.
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