ArticleThe EMBO journal1995
Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 115 citations in OpenAlex.
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- Single and Combined Methods to Specifically or Bulk-Purify RNA-Protein Complexes.Biomolecules · 2020Review
- The Ribosome as a Switchboard for Bacterial Stress Response.Frontiers in microbiology · 2020Review
- Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein-RNA Interactions.Molecular & cellular proteomics : MCP · 2015Article
- Identification of Novel RNA-Protein Contact in Complex of Ribosomal Protein S7 and 3'-Terminal Fragment of 16S rRNA in E. coli.Acta naturae · 2012Article
- Metalloproteins and the pyrite-based origin of life: a critical assessment.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2011Article
- Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion.Molecular & cellular proteomics : MCP · 2010Article
- Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles.RNA (New York, N.Y.) · 2010Review
- Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription.Viruses · 2009Article
- Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4.The Journal of biological chemistry · 2009Article
- Nested Arg-specific bifunctional crosslinkers for MS-based structural analysis of proteins and protein assemblies.Analytica chimica acta · 2008Article
- Application of fractional mass for the identification of peptide-oligonucleotide cross-links by mass spectrometry.Journal of mass spectrometry : JMS · 2008Article
- Developing limited proteolysis and mass spectrometry for the characterization of ribosome topography.Journal of the American Society for Mass Spectrometry · 2007Article
- Improved identification of enriched peptide RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry.Nucleic acids research · 2007Article
- A protein extension to shorten RNA: elongated elongation factor-Tu recognizes the D-arm of T-armless tRNAs in nematode mitochondria.The Biochemical journal · 2006Article
- Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles.RNA (New York, N.Y.) · 2005Article
- Modulation of zinc- and cobalt-binding affinities through changes in the stability of the zinc ribbon protein L36.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2005Article
- A novel strategy for the identification of protein-DNA contacts by photocrosslinking and mass spectrometry.Nucleic acids research · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated protein-rRNA cross-links formed in 30S and 50S ribosomal subunits of Escherichia coli and Bacillus stearothermophilus at the molecular level using UV and 2-iminothiolane as cross-linking agents. We identified amino acids cross-linked to rRNA for 13 ribosomal proteins from these organisms, namely derived from S3, S4, S7, S14, S17, L2, L4, L6, L14, L27, L28, L29 and L36. Several other peptide stretches cross-linked to rRNA have been sequenced in which no direct cross-linked amino acid could be detected. The cross-linked amino acids are positioned within loop domains carrying RNA binding features such as conserved basic and aromatic residues. One of the cross-linked peptides in ribosomal protein S3 shows a common primary sequence motif--the KH motif--directly involved in interaction with rRNA, and the cross-linked amino acid in ribosomal protein L36 lies within the zinc finger-like motif of this protein. The cross-linked amino acids in ribosomal proteins S17 and L6 prove the proposed RNA interacting site derived from three-dimensional models. A comparison of our structural data with mutations in ribosomal proteins that lead to antibiotic resistance, and with those from protein-antibiotic cross-linking experiments, reveals functional implications for ribosomal proteins that interact with rRNA.
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