ArticleThe EMBO journal1995
Two group I ribozymes with different functions in a nuclear rDNA intron.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Telomere-to-telomere characterization of rDNA chromosome in the myxomycete Didymium iridis.BMC molecular and cell biology · 2026Article
- Ribocentre: a database of ribozymes.Nucleic acids research · 2023Article
- Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns.Mobile DNA · 2022Article
- Lariat capping as a tool to manipulate the 5' end of individual yeast mRNA species in vivo.RNA (New York, N.Y.) · 2017Article
- Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock.Molecules (Basel, Switzerland) · 2016Article
- RNA circularization strategies in vivo and in vitro.Nucleic acids research · 2015Review
- Molecular characterization of a new member of the lariat capping twin-ribozyme introns.Mobile DNA · 2014Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA.RNA (New York, N.Y.) · 2009Article
- Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes.The EMBO journal · 2008Article
- The recent transfer of a homing endonuclease gene.Nucleic acids research · 2005Article
- Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA.Nucleic acids research · 2005Article
- The ability to form full-length intron RNA circles is a general property of nuclear group I introns.RNA (New York, N.Y.) · 2003Article
- Flanking sequences with an essential role in hydrolysis of a self-cleaving group I-like ribozyme.Nucleic acids research · 2000Article
- Expression of the Naegleria intron endonuclease is dependent on a functional group I self-cleaving ribozyme.RNA (New York, N.Y.) · 2000Article
- Functional alpha-fragment of beta-galactosidase can be expressed from the mobile group I intron PpLSU3 embedded in yeast pre-ribosomal RNA derived from the chromosomal rDNA locus.Nucleic acids research · 2000Article
- In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.The EMBO journal · 1999Article
- In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.RNA (New York, N.Y.) · 1998Article
- I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.Molecular and cellular biology · 1998Article
- Group I-like ribozymes with a novel core organization perform obligate sequential hydrolytic cleavages at two processing sites.RNA (New York, N.Y.) · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DiSSU1, a mobile intron in the nuclear rRNA gene of Didymium iridis, was previously reported to contain two independent catalytic RNA elements. We have found that both catalytic elements, renamed GIR1 and GIR2, are group I ribozymes, but with differing functionality. GIR2 carries out the several reactions associated with self-splicing. GIR1 carries out a hydrolysis reaction at an internal processing site (IPS-1). These conclusions are based on the catalytic properties of RNAs transcribed in vitro. Mutation of the P7 pairing segment of GIR2 abrogated self-splicing, while mutation of P7 in GIR1 abrogated hydrolysis at the IPS-1. Much of the P2 stem and all of the associated loop could be deleted without effect on self-splicing. These results are accounted for by a secondary structure model, in which a long P2 pairing segment brings the 5' splice site to the GIR2 catalytic core. GIR1 is the smallest natural group I ribozyme yet reported and is the first example of a group I ribozyme whose presumptive biological function is hydrolysis. We hypothesize that GIR1-mediated cleavage of the excised intron RNA functions in the generation and expression of the mRNA for the intron-encoded endonuclease I-DirI.
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