ArticleThe EMBO journal1995
Signal transduction by the alpha 6 beta 4 integrin: distinct beta 4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
76 citing papers in PubMed, 239 citations in OpenAlex.
- Structure, function and usage of integrin alpha 6 (CD49f): A comprehensive review.Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia · 2026Review
- Double-sided niche regulation in skin stem cell and cancer: mechanisms and clinical applications.Molecular cancer · 2025Review
- Regulation of Kinase Signaling Pathways by α6β4-Integrins and Plectin in Prostate Cancer.Cancers · 2022Review
- Mechanical regulation of signal transduction in angiogenesis.Frontiers in cell and developmental biology · 2022Review
- EGFR-dependent tyrosine phosphorylation of integrin β4 is not required for downstream signaling events in cancer cell lines.Scientific reports · 2021Article
- Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells.Scientific reports · 2020Article
- Insights into the Shc Family of Adaptor Proteins.Journal of molecular signaling · 2017Article
- SYK interaction with ITGβ4 suppressed by Epstein-Barr virus LMP2A modulates migration and invasion of nasopharyngeal carcinoma cells.Oncogene · 2015Article
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Focal Contact and Hemidesmosomal Proteins in Keratinocyte Migration and Wound Repair.Advances in wound care · 2014Review
- Critical role of S1PR1 and integrin β4 in HGF/c-Met-mediated increases in vascular integrity.The Journal of biological chemistry · 2013Article
- Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae.PloS one · 2013Article
- A Dictyostelium SH2 adaptor protein required for correct DIF-1 signaling and pattern formation.Developmental biology · 2011Article
- Comparative study of the dynamics of focal contacts in live epithelial and mesenchymal cells.Medical molecular morphology · 2011Article
- The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1.The EMBO journal · 2010Article
- Endothelial cell-laminin interaction: modulation of LDH expression involves alpha6beta4 integrin-FAK-p38MAPK pathway.Glycoconjugate journal · 2009Article
- A novel role of Shc adaptor proteins in steroid hormone-regulated cancers.Endocrine-related cancer · 2009Review
- Spatial signaling networks converge at the adaptor protein Shc.Cell cycle (Georgetown, Tex.) · 2009Article
- ShcA signalling is essential for tumour progression in mouse models of human breast cancer.The EMBO journal · 2008Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
We have examined the mechanism of signal transduction by the hemidesmosomal integrin alpha 6 beta 4, a laminin receptor involved in morphogenesis and tumor progression. Immunoprecipitation and immune complex kinase assays indicated that antibody- or laminin-induced ligation of alpha 6 beta 4 causes tyrosine phosphorylation of the beta 4 subunit in intact cells and that this event is mediated by a protein kinase(s) physically associated with the integrin. Co-immunoprecipitation and GST fusion protein binding experiments showed that the adaptor protein Shc forms a complex with the tyrosine-phosphorylated beta 4 subunit. Shc is then phosphorylated on tyrosine residues and recruits the adaptor Grb2, thereby potentially linking alpha 6 beta 4 to the ras pathway. The beta 4 subunit was found to be phosphorylated at multiple tyrosine residues in vivo, including a tyrosine-based activation motif (TAM) resembling those found in T and B cell receptors. Phenylalanine substitutions at the beta 4 TAM disrupted association of alpha 6 beta 4 with hemidesmosomes, but did not interfere with tyrosine phosphorylation of Shc and recruitment of Grb2. These results indicate that signal transduction by the alpha 6 beta 4 integrin is mediated by an associated tyrosine kinase and that phosphorylation of distinct sites in the beta 4 tail mediates assembly of the hemidesmosomal cytoskeleton and recruitment of Shc/Grb2.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.