ArticleThe EMBO journal1995
A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- AraC Functional Suppressors of Mutations in the C-Terminal Domain of the RpoA Subunit of theMicroorganisms · 2024Article
- Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract.Infection and immunity · 2020Article
- Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography.The protein journal · 2019Review
- Escherichia coli SecG is required for residual export mediated by mutant signal sequences and for SecY-SecE complex stability.Journal of bacteriology · 2015Article
- How does Tremblaya princeps get essential proteins from its nested partner Moranella endobia in the Mealybug Planoccocus citri?PloS one · 2013Article
- The bacterial Sec-translocase: structure and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Mapping of the SecA·SecY and SecA·SecG interfaces by site-directed in vivo photocross-linking.The Journal of biological chemistry · 2011Article
- Topologically fixed SecG is fully functional.Journal of bacteriology · 2006Article
- A novel class of secA alleles that exert a signal-sequence-dependent effect on protein export in Escherichia coli.Genetics · 2002Article
- Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli.Journal of bacteriology · 2001Article
- SecG function and phospholipid metabolism in Escherichia coli.Journal of bacteriology · 2001Article
- Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion.The EMBO journal · 2000Article
- Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter.Journal of bacteriology · 1999Article
- Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.Journal of bacteriology · 1999Article
- Roles of SecG in ATP- and SecA-dependent protein translocation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Linkage map of Escherichia coli K-12, edition 10: the traditional map.Microbiology and molecular biology reviews : MMBR · 1998Review
- The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events.The EMBO journal · 1997Article
- Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The signal sequence of the murine serine protease inhibitor PAI-2 promotes alkaline phosphatase export to the E. coli periplasm. However, high level expression of this chimeric protein interferes with cell growth. Since most suppressors of this toxic phenotype map to secA and secY, growth arrest results from a defective interaction of the chimeric protein with the export machinery. We have characterized suppressors which map in secG, a newly defined gene of the export machinery. All single amino acid substitutions map to three adjacent codons. These secG mutants have a weak Sec phenotype, as determined by their effect on export mediated by wild-type and mutant signal sequences. Whilst a secG disruption allele also confers a weak Sec phenotype, it does not suppress the toxicity of the chimeric protein. This difference results from a selective effect of the secG suppressors on the kinetics of export mediated by the PAI-2 signal sequence. Using a malE signal sequence mutant, which has a Mal-phenotype in secG mutant strains, we have isolated extragenic Mal+ suppressors. Most suppressors map to secY, and several are allele-specific. Finally, SecG overexpression accelerates the kinetics of protein export, suggesting that there are two types of functional translocation complexes: with or without SecG.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.