ArticleThe EMBO journal1995
The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- Optimization of Expression and Thermostability of Terminal Deoxynucleotidyl Transferase Through Iterative Mutagenesis and Computational Design.Applied biochemistry and biotechnology · 2026Article
- Research progress on the relationship between the TdT gene and autoimmune diseases.Frontiers in immunology · 2026Review
- Deep thermal profiling for detection of functional proteoform groups.Nature chemical biology · 2023Article
- Insight into the mechanism of DNA synthesis by human terminal deoxynucleotidyltransferase.Life science alliance · 2022Article
- Variations in nuclear localization strategies among pol X family enzymes.Traffic (Copenhagen, Denmark) · 2018Article
- DNA polymerase β contains a functional nuclear localization signal at its N-terminus.Nucleic acids research · 2017Article
- Decision-making during NHEJ: a network of interactions in human Polμ implicated in substrate recognition and end-bridging.Nucleic acids research · 2014Article
- Expansion of the preimmune antibody repertoire by junctional diversity in Bos taurus.PloS one · 2014Article
- LenVarDB: database of length-variant protein domains.Nucleic acids research · 2014Article
- Terminal deoxynucleotidyl transferase: the story of a misguided DNA polymerase.Biochimica et biophysica acta · 2010Review
- A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis.Nucleic acids research · 2006Article
- Terminal deoxynucleotidyl transferases from elasmobranchs reveal structural conservation within vertebrates.Immunogenetics · 2003Article
- Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase.The EMBO journal · 2002Article
- Highly frequent frameshift DNA synthesis by human DNA polymerase mu.Molecular and cellular biology · 2001Article
- Promotion of cell cycle progression by basic helix-loop-helix E2A.Molecular and cellular biology · 2001Article
- Ku80 is required for addition of N nucleotides to V(D)J recombination junctions by terminal deoxynucleotidyl transferase.Nucleic acids research · 2001Article
- The long isoform of terminal deoxynucleotidyl transferase enters the nucleus and, rather than catalyzing nontemplated nucleotide addition, modulates the catalytic activity of the short isoform.The Journal of experimental medicine · 2001Article
- DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells.The EMBO journal · 2000Article
- High-level expression of murine terminal deoxynucleotidyl transferase in Escherichia coli grown at low temperature and overexpressing argU tRNA.Molecular biotechnology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two alternatively spliced terminal deoxynucleotidyl transferase transcripts, TdTS and TdTL which code respectively for proteins of 509 and 529 amino acids have been previously identified in the mouse thymus. Here we show that the same two transcripts are also present in B lineage cells from bone marrow. In addition we demonstrate that the corresponding 20 amino acid insertion found near the carboxy-terminal end of TdTL significantly alters the function of the enzyme. In contrast to TdTS, TdTL does not catalyse N region insertions at the recombination junction of a V(D)J site-specific recombination substrate. In an attempt to explain the lack of N region insertions we have characterized the different parameters which distinguish the two isoforms of TdT. Examination of transfected cell extracts revealed a reduced capacity of TdTL to add nucleotides to the 3' end of DNA, consistent with a lower terminal transferase activity. Furthermore, the half-life of the TdTL protein in these cells is 2-fold shorter than that of TdTS. Finally, despite the fact that TdTL has the same nuclear localization signal as TdTS, the cellular localization of the two isoforms was strikingly different. In contrast to nuclear TdTS, TdTL was found exclusively in the cytoplasm. All these characteristics could contribute to the functional difference between the two isoforms of TdT. However, the subcellular localization of TdTL on its own can account for its inability to add N regions.
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