ArticleMolecular biotechnology1995
Rapid optimization of electroporation conditions for plant cells, protoplasts, and pollen.
Article in Molecular biotechnology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Robust and ReproducibleFrontiers in plant science · 2020Article
- Highly integrated microfluidic device for cell pairing, fusion and culture.Biomicrofluidics · 2019Article
- Rice GROWTH UNDER DROUGHT KINASE is required for drought tolerance and grain yield under normal and drought stress conditions.Plant physiology · 2014Article
- A geminivirus AYVV-derived shuttle vector for tobacco BY2 cells.Plant cell reports · 2004Article
- Plant-based model for the visual evaluation of electroporated area after irreversible electroporation and its comparison to in-vivo animal data.Science progressArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The optimization of electroporation conditions for maximal uptake of DNA during direct gene transfer experiments is critical to achieve high levels of gene expression in transformed plant cells. Two stains, trypan blue and fluorescein diacetate, have been applied to optimize electroporation conditions for three plant cell types, using different square wave and exponential wave electroporation devices. The different cell types included protoplasts from tobacco, a stable mixotrophic suspension cell culture from soybean with intact cell walls, and germinating pollen from alfalfa and tobacco. Successful electroporation of each of these cell types was obtained, even in the presence of an intact cell wall when conditions were optimized for the electroporation pulse. The optimal field strength for each of these cells differs, protoplasts having the lowest optimal pulse field strength, followed by suspension cells and finally germinating pollen requiring the strongest electroporation pulse. A rapid procedure is described for optimizing electroporation parameters using different types of cells from different plant sources.
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