ArticleThe Plant cell1995
The initiation codon determines the efficiency but not the site of translation initiation in Chlamydomonas chloroplasts.
Article in The Plant cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Plastome evolution in annual Brachypodium species reveals widespread heteroplasmy and chloroplast capture, lineage-specific codon usage bias, and low positive selection.BMC plant biology · 2026Article
- The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii.The Plant cell · 2021Article
- ThePlant physiology · 2019Article
- Dynamics of Chloroplast Translation during Chloroplast Differentiation in Maize.PLoS genetics · 2016Article
- Complete sequence and comparative analysis of the chloroplast genome of coconut palm (Cocos nucifera).PloS one · 2013Article
- Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Article
- Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.Plant molecular biology · 2006Article
- Analysis of developing maize plastids reveals two mRNA stability classes correlating with RNA polymerase type.EMBO reports · 2004Article
- Flagellar radial spoke protein 2 is a calmodulin binding protein required for motility in Chlamydomonas reinhardtii.Eukaryotic cell · 2004Article
- Surprising features of plastid ndhD transcripts: addition of non-encoded nucleotides and polysome association of mRNAs with an unedited start codon.Nucleic acids research · 2004Article
- Zeaxanthin accumulation in the absence of a functional xanthophyll cycle protects Chlamydomonas reinhardtii from photooxidative stress.The Plant cell · 2003Article
- In vivo evidence for the prokaryotic model of extended codon-anticodon interaction in translation initiation.The EMBO journal · 2003Article
- Involvement of state transitions in the switch between linear and cyclic electron flow in Chlamydomonas reinhardtii.EMBO reports · 2002Article
- A role for initiation codon context in chloroplast translation.The Plant cell · 2001Article
- Mobile self-splicing group I introns from the psbA gene of Chlamydomonas reinhardtii: highly efficient homing of an exogenous intron containing its own promoter.Molecular and cellular biology · 2001Article
- The sequence and secondary structure of the 3'-UTR affect 3'-end maturation, RNA accumulation, and translation in tobacco chloroplasts.Plant molecular biology · 2000Article
- cis- and trans-Acting determinants for translation of psbD mRNA in Chlamydomonas reinhardtii.Molecular and cellular biology · 2000Article
- Evidence for a role of ClpP in the degradation of the chloroplast cytochrome b(6)f complex.The Plant cell · 2000Article
- A nuclear gene in maize required for the translation of the chloroplast atpB/E mRNA.The Plant cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study translation initiation in Chlamydomonas chloroplasts, we mutated the initiation codon AUG to AUU, ACG, ACC, ACU, and UUC in the chloroplast petA gene, which encodes cytochrome f of the cytochrome b6/f complex. Cytochrome f accumulated to detectable levels in all mutant strains except the one with a UUC codon, but only the mutant with an AUU codon grew well at 24 degrees C under conditions that require photosynthesis. Because no cytochrome f was detectable in the UUC mutant and because each mutant that accumulated cytochrome f did so at a different level, we concluded that any residual translation probably initiates at the mutant codon. As a further demonstration that alternative initiation sites are not used in vivo, we introduced in-frame UAA stop codons immediately downstream or upstream or in place of the initiation codon. Stop codons at or downstream of the initiation codon prevented accumulation of cytochrome f, whereas the one immediately upstream of the initiation codon had no effect on the accumulation of cytochrome f. These results suggest that an AUG codon is not required to specify the site of translation initiation in chloroplasts but that the efficiency of translation initiation depends on the identity of the initiation codon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.