ArticleProceedings of the National Academy of Sciences of the United States of America1995
Hypertonicity activates nonselective cation channels in mouse cortical collecting duct cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 70 citations in OpenAlex.
- Cell death induction and protection by activation of ubiquitously expressed anion/cation channels. Part 3: the roles and properties of TRPM2 and TRPM7.Frontiers in cell and developmental biology · 2023Review
- Mechanical stretch increases Kv1.5 current through an interaction between the S1-S2 linker and N-terminus of the channel.The Journal of biological chemistry · 2020Article
- Inhibition of erythrocyte "apoptosis" by catecholamines.Naunyn-Schmiedeberg's archives of pharmacology · 2005Article
- Ion channels in cell proliferation and apoptotic cell death.The Journal of membrane biology · 2005Review
- Cation channels, cell volume and the death of an erythrocyte.Pflugers Archiv : European journal of physiology · 2003Review
- Ca(2+)- and voltage-dependent gating of Ca(2+)- and ATP-sensitive cationic channels in brain capillary endothelium.Biophysical journal · 2003Article
- Inhibition of erythrocyte cation channels and apoptosis by ethylisopropylamiloride.Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- A hypertonicity-activated nonselective conductance in single proximal tubule cells isolated from mouse kidney.The Journal of membrane biology · 2003Article
- The heterodimeric amino acid transporter 4F2hc/LAT1 is associated in Xenopus oocytes with a non-selective cation channel that is regulated by the serine/threonine kinase sgk-1.The Journal of physiology · 2000Article
- An intracellular ATP-activated, calcium-permeable conductance on the basolateral membrane of single renal proximal tubule cells isolated from Rana temporaria.The Journal of physiology · 2000Article
- Calcium-, voltage- and osmotic stress-sensitive currents in Xenopus oocytes and their relationship to single mechanically gated channels.The Journal of physiology · 2000Article
- Cell swelling activates ATP-dependent voltage-gated chloride channels in M-1 mouse cortical collecting duct cells.The Journal of general physiology · 1996Article
- Amiloride-sensitive sodium channels in confluent M-1 mouse cortical collecting duct cells.The Journal of membrane biology · 1995Article
- A calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells.The Journal of membrane biology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the effect of cell shrinkage on whole-cell currents of M-1 mouse cortical collecting duct cells. Addition of 100 mM sucrose to an isotonic NaCl bath solution induced cell shrinkage and increased whole-cell currents within 5-10 min by approximately 12-fold. The effect was reversible upon return to isotonic solution and could also be elicited by adding 100 mM urea or 50 mM NaCl. Replacement of bath Na+ by K+, Cs+, Li+, or Rb+ did not significantly affect the stimulated inward current, but replacement by N-methyl-D-glucamine reduced it by 88.1 +/- 1.3% (n = 34); this demonstrates that hypertonicity activates a nonselective alkali cation conductance. The activation was independent of extra- and intracellular Ca2+, but 1 or 10 mM ATP in the pipette suppressed it in a concentration-dependent manner, indicating that intracellular ATP levels may modulate the degree of channel activation. Flufenamic acid (0.1 mM) and gadolinium (0.1 mM) inhibited the stimulated current by 68.7 +/- 5.9% (n = 9) and 32.4 +/- 11.7% (n = 6), respectively, whereas 0.1 mM amiloride had no significant effect. During the early phase of hypertonic stimulation single-channel transitions could be detected in whole-cell current recordings, and a gradual activation of 30 and more individual channels with a single-channel conductance of 26.7 +/- 0.4 pS (n = 29) could be resolved. Thus, we identified the nonselective cation channel underlying the shrinkage-induced whole-cell conductance that may play a role in volume regulation.
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