ArticleMolecular biology of the cell1995
Membrane protein trafficking through the common apical endosome compartment of polarized Caco-2 cells.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Differential compartmentalization of BMP4/NOGGIN requires NOGGIN trans-epithelial transport.Developmental cell · 2021Article
- Importance of endocytic pathways in liver function and disease.Comprehensive Physiology · 2014Review
- Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro.International journal of cell biology · 2010Article
- Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains.Molecular biology of the cell · 2007Article
- Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment.Molecular biology of the cell · 2004Article
- Myosin vb is associated with plasma membrane recycling systems.Molecular biology of the cell · 2001Article
- Sorting of membrane and fluid at the apical pole of polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2000Article
- Polarized sphingolipid transport from the subapical compartment: evidence for distinct sphingolipid domains.Molecular biology of the cell · 1999Article
- Vectorial function of major histocompatibility complex class II in a human intestinal cell line.Immunology · 1999Article
- Evidence for apical endocytosis in polarized hepatic cells: phosphoinositide 3-kinase inhibitors lead to the lysosomal accumulation of resident apical plasma membrane proteins.The Journal of cell biology · 1999Article
- Trafficking of Shigella lipopolysaccharide in polarized intestinal epithelial cells.The Journal of cell biology · 1999Article
- Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 1999Article
- Sorting mechanisms regulating membrane protein traffic in the apical transcytotic pathway of polarized MDCK cells.The Journal of cell biology · 1998Article
- Highly polarized HLA class II antigen processing and presentation by human intestinal epithelial cells.The Journal of clinical investigation · 1998Article
- Apical plasma membrane proteins and endolyn-78 travel through a subapical compartment in polarized WIF-B hepatocytes.The Journal of cell biology · 1998Article
- Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells.The Journal of cell biology · 1998Article
- Identification of a somatodendritic targeting signal in the cytoplasmic domain of the transferrin receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells.The Journal of cell biology · 1997Article
- Apical and basolateral endosomes of MDCK cells are interconnected and contain a polarized sorting mechanism.The Journal of cell biology · 1996Article
- Reconstitution of transcytosis in SLO-permeabilized MDCK cells: existence of an NSF-dependent fusion mechanism with the apical surface of MDCK cells.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By raising monoclonal antibodies to the apical surface of Caco-2 cells we have identified a membrane protein (p100) that internalizes and recycles constitutively between the apical plasma membrane and endosomes in the apical cytoplasm. By applying tracers bound to the transferrin receptor, which internalizes and recycles back to the basolateral border, we demonstrate that the apical endosomes containing p100 include a subset of multivesticular bodies (MVB), which are also accessible to proteins arriving from the basolateral endosome. Tracers bound to EGF receptors and alpha-2-macroglobulin, which internalize from the basolateral border and are degraded, probably in lysosomes, also pass through the p100-containing MVB. These studies therefore suggest that the apical cytoplasm of Caco-2 cells contains a population of MVB capable of receiving membrane proteins trafficking in from both apical and basolateral borders and then routing them to a variety of cell surface and intracellular destinations. The differential distribution of apical and basolateral tracers within the 50-nm-diameter tubules connected to these p100-positive apical MVB suggests that the destination of proteins trafficking from the MVB back to apical and basolateral surfaces is determined by the tubules to which they gain access.
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