ArticleThe Journal of cell biology1995
Biogenesis of synaptic vesicles in vitro.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- On the Computing Potential of Intracellular Vesicles.PloS one · 2015Article
- A distinct trans-Golgi network subcompartment for sorting of synaptic and granule proteins in neurons and neuroendocrine cells.Journal of cell science · 2011Article
- A fluorescence-based in vitro assay for investigating early endosome dynamics.Nature protocols · 2010Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Biochemical characterization of the coating mechanism of the endosomal donor compartment of synaptic vesicles.Neurochemical research · 2004Article
- The dopamine transporter constitutively internalizes and recycles in a protein kinase C-regulated manner in stably transfected PC12 cell lines.The Journal of biological chemistry · 2003Article
- Birbeck granules are subdomains of endosomal recycling compartment in human epidermal Langerhans cells, which form where Langerin accumulates.Molecular biology of the cell · 2002Article
- Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells.Molecular biology of the cell · 2001Article
- The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Identification of discrete classes of endosome-derived small vesicles as a major cellular pool for recycling membrane proteins.Molecular biology of the cell · 2001Article
- Neurosecretory granule formation in ligated axons: additional arguments for a local differentiation from a Golgi apparatus extension.The Histochemical journal · 2001Article
- Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells.Molecular biology of the cell · 1999Article
- Secretagogue-triggered transfer of membrane proteins from neuroendocrine secretory granules to synaptic-like microvesicles.Molecular biology of the cell · 1999Article
- A complex web of signal-dependent trafficking underlies the triorganellar distribution of P-selectin in neuroendocrine PC12 cells.The Journal of cell biology · 1999Article
- Neuroendocrine synaptic vesicles are formed in vitro by both clathrin-dependent and clathrin-independent pathways.The Journal of cell biology · 1998Article
- Vesiculation and sorting from PC12-derived endosomes in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes.The Journal of cell biology · 1998Article
- GLUT4 and transferrin receptor are differentially sorted along the endocytic pathway in CHO cells.The Journal of cell biology · 1998Article
- A signaling organelle containing the nerve growth factor-activated receptor tyrosine kinase, TrkA.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- ADP ribosylation factor 1 is required for synaptic vesicle budding in PC12 cells.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Synaptic vesicles are synthesized at a rapid rate in nerve terminals to compensate for their rapid loss during neurotransmitter release. Their biogenesis involves endocytosis of synaptic vesicle membrane proteins from the plasma membrane and requires two steps, the segregation of synaptic vesicle membrane proteins from other cellular proteins, and the packaging of those unique proteins into vesicles of the correct size. By labeling an epitope-tagged variant of a synaptic vesicle protein, VAMP (synaptobrevin), at the cell surface of the neuroendocrine cell line PC12, synaptic vesicle biogenesis could be followed with considerable precision, quantitatively and kinetically. Epitope-tagged VAMP was recovered in synaptic vesicles within a few minutes of leaving the cell surface. More efficient targeting was obtained by using the VAMP mutant, del 61-70. Synaptic vesicles did not form at 15 degrees C although endocytosis still occurred. Synaptic vesicles could be generated in vitro from a homogenate of cells labeled at 15 degrees C. The newly formed vesicles are identical to those formed in vivo in their sedimentation characteristics, the presence of the synaptic vesicle protein synaptophysin, and the absence of detectable transferrin receptor. Brain, but not fibroblast cytosol, allows vesicles of the correct size to form. Vesicle formation is time and temperature-dependent, requires ATP, is calcium independent, and is inhibited by GTP-gamma S. Thus, two key steps in synaptic vesicle biogenesis have been reconstituted in vitro, allowing direct analysis of the proteins involved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.