ArticleBritish journal of cancer1995
Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.
Article in British journal of cancer, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 136 citations in OpenAlex.
- A systematic review of the role of human papilloma virus (HPV) testing within a cervical screening programme: summary and conclusions.British journal of cancer · 2000Pooled it
- Genotyping HPV 16 and 18 in oral and oropharyngeal cancer: uncovering HIV as a key risk factor in a Ugandan cohort.Infectious agents and cancer · 2025Article
- Human papillomavirus vaccine effect against human papillomavirus infection in Rwanda: evidence from repeated cross-sectional cervical-cell-based surveys.The Lancet. Global health · 2023Article
- Impact of Human Papillomavirus Vaccination, Rwanda and Bhutan.Emerging infectious diseases · 2021Review
- Human papillomavirus genotypes in cervical and other HPV-related anogenital cancer in Rwanda, according to HIV status.International journal of cancer · 2020Article
- Evaluation of human-papillomavirus testing and visual inspection for cervical cancer screening in Rwanda.BMC women's health · 2018Article
- Frequency of HPV in oral cavity squamous cell carcinoma.BMC cancer · 2018Article
- Evaluation of cytology versus human papillomavirus-based cervical cancer screening algorithms in Bhutan.Oncotarget · 2017Article
- Evaluation of the performance of Human Papillomavirus testing in paired urine and clinician-collected cervical samples among women aged over 30 years in Bhutan.Virology journal · 2017Article
- Detection of Human Papillomavirus Genotypes and Epstein-Barr Virus in Nasopharyngeal Carcinomas at the Korle-Bu Teaching Hospital, Ghana.TheScientificWorldJournal · 2017Article
- Comparison of Two Widely Used Human Papillomavirus Detection and Genotyping Methods, GP5+/6+-Based PCR Followed by Reverse Line Blot Hybridization and Multiplex Type-Specific E7-Based PCR.Journal of clinical microbiology · 2016Article
- Human papillomavirus infection in Rwanda at the moment of implementation of a national HPV vaccination programme.BMC infectious diseases · 2016Article
- Prevalence of genital human papillomavirus among rural and urban populations in southern Yunnan province, China.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2016Article
- Distribution of Carcinogenic Human Papillomavirus Genotypes and Association to Cervical Lesions among Women in Fez (Morocco).PloS one · 2016Article
- First Detection of Human Papillomaviruses and Human Polyomaviruses in River Waters in Italy.Food and environmental virology · 2015Article
- Human papillomavirus infection in Bhutan at the moment of implementation of a national HPV vaccination programme.BMC infectious diseases · 2014Article
- Clinical validation of the HPV-risk assay, a novel real-time PCR assay for detection of high-risk human papillomavirus DNA by targeting the E7 region.Journal of clinical microbiology · 2014Article
- Molecular diagnostic alterations in squamous cell carcinoma of the head and neck and potential diagnostic applications.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2014Review
- Prevalence of HPV infection and other risk factors in a Fijian population.Infectious agents and cancer · 2014Article
- HPV E6/E7 RNA in situ hybridization signal patterns as biomarkers of three-tier cervical intraepithelial neoplasia grade.PloS one · 2014Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/silica beads method, which appeared superior, revealed a human papillomavirus (HPV) general primer-mediated PCR sensitivity of 50-500 copies of HPV 16 per sample using dilutions of fixed and stained Siha cells. Application to archival cervical smears (n = 116) revealed that the yield and size of amplifiable DNA decreases with storage time. The longer the storage time, the more repetitions of the whole procedure, including the lysis step, were required to extract sufficient amplifiable DNA. In this way, an overall beta-globin PCR positivity for 98% of the smears was reached. Further analysis revealed that a maximum size of 200 bp could be amplified from smears stored for up to 9 years. The method was validated by demonstrating by PCR the same HPV types in archival smears and corresponding cervical biopsies of cervical cancer patients. In conclusion, the GTC/silica beads method appears suitable to process archival cervical smears for HPV detection by PCR. provided that stepwise adjustments are made until beta-globin PCR positivity is obtained and primers are chosen which amplify a maximum of about 200 bp.
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