ArticleNucleic acids research1995
Purification and properties of human DNA helicase VI.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Structural and functional characterization of human SLFN14.Nucleic acids research · 2025Article
- Article
- Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A.Nucleic acids research · 2004Article
- Identification of human DNA helicase V with the far upstream element-binding protein.Nucleic acids research · 2001Article
- A chloroplast DNA helicase II from pea that prefers fork-like replication structuresPlant physiology · 1998Article
- Characterization of Werner syndrome protein DNA helicase activity: directionality, substrate dependence and stimulation by replication protein A.Nucleic acids research · 1998Article
- Identification of DNA replication and cell cycle proteins that interact with PCNA.Nucleic acids research · 1997Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel ATP-dependent DNA unwinding enzyme, called human DNA helicase VI (HDH VI), was purified to apparent homogeneity from HeLa cells and characterized. From 327 g of cultured cells, 0.44 mg of pure enzyme was recovered, free of DNA polymerase, ligase, topoisomerase, nicking and nuclease activities. The enzyme behaves as a monomer having an M(r) of 128 kDa, whether determined with SDS-PAGE, or in native conditions. Photoaffinity labelling with [alpha-32P]ATP labelled the 128 kDa protein. Only ATP or dATP hydrolysis supports the unwinding activity for which a divalent cation (Mg2+ > Mn2+) is required. HDH VI unwinds exclusively DNA duplexes with an annealed portion < 32 bp and prefers a replication fork-like structure of the substrate. It cannot unwind blunt-end duplexes and is inactive also on DNA-RNA or RNA-RNA hybrids. HDH VI unwinds DNA unidirectionally by moving in the 3' to 5' direction along the bound strand.
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