ArticleProceedings of the National Academy of Sciences of the United States of America1995
Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 103 citations in OpenAlex.
- An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus.Cancer cell international · 2014Article
- LC/LC-MS/MS of an innovative prostate human epithelial cancer (PHEC) in vitro model system.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2012Article
- iTRAQ identification of candidate serum biomarkers associated with metastatic progression of human prostate cancer.PloS one · 2012Article
- An evaluation of molecular markers for improved detection of breast cancer metastases in sentinel nodes.Journal of clinical pathology · 2006Article
- Identification of calcium- and nitric oxide-regulated genes by differential analysis of library expression (DAzLE).Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Up-regulation of expression of translation factors--a novel molecular mechanism for cadmium carcinogenesis.Molecular and cellular biochemistry · 2004Article
- Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The role of protein elongation factor eEF1A2 in ovarian cancer.Reproductive biology and endocrinology : RB&E · 2003Review
- Not just for housekeeping: protein initiation and elongation factors in cell growth and tumorigenesis.Journal of molecular medicine (Berlin, Germany) · 2003Review
- PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks.Oncogene · 2001Article
- Investigation into the mechanism of the loss of laminin 5 (alpha3beta3gamma2) expression in prostate cancer.The American journal of pathology · 2001Article
- Comparison of human telomerase reverse transcriptase messenger RNA and telomerase activity as urine markers for diagnosis of bladder carcinoma.Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology · 2000Article
- RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer.The American journal of pathology · 1999Article
- Reciprocal subtraction differential RNA display: an efficient and rapid procedure for isolating differentially expressed gene sequences.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Antisense inhibition of the PTI-1 oncogene reverses cancer phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Molecular cancer vaccines: Tumor therapy using antigen-specific immunizations.Pathology oncology research : POR · 1997Article
- Isolation of a novel gene showing reduced expression in metastatic colorectal carcinoma cell lines and carcinomas.Japanese journal of cancer research : Gann · 1997Article
- The melanoma differentiation associated gene mda-7 suppresses cancer cell growth.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Elucidating the relevant genomic changes mediating development and evolution of prostate cancer is paramount for effective diagnosis and therapy. A putative dominant-acting nude mouse prostatic carcinoma tumor-inducing gene, PTI-1, has been cloned that is expressed in patient-derived human prostatic carcinomas but not in benign prostatic hypertrophy or normal prostate tissue. PTI-1 was detected by cotransfecting human prostate carcinoma DNA into CREF-Trans 6 cells, inducing tumors in nude mice, and isolating genes displaying increased expression in tumor-derived cells by using differential RNA display (DD). Screening a human prostatic carcinoma (LNCaP) cDNA library with a 214-bp DNA fragment found by DD permitted the cloning of a full-length 2.0-kb PTI-1 cDNA. Sequence analysis indicates that PTI-1 is a gene containing a 630-bp 5' sequence and a 3' sequence homologous to a truncated and mutated form of human elongation factor 1 alpha. In vitro translation demonstrates that the PTI-1 cDNA encodes a predominant approximately 46-kDa protein. Probing Northern blots with a DNA fragment corresponding to the 5' region of PTI-1 identifies multiple PTI-1 transcripts in RNAs from human carcinoma cell lines derived from the prostate, lung, breast, and colon. In contrast, PTI-1 RNA is not detected in human melanoma, neuroblastoma, osteosarcoma, normal cerebellum, or glioblastoma multiforme cell lines. By using a pair of primers recognizing a 280-bp region within the 630-bp 5' PTI-1 sequence, reverse transcription-PCR detects PTI-1 expression in patient-derived prostate carcinomas but not in normal prostate or benign hypertrophic prostate tissue. In contrast, reverse transcription-PCR detects prostate-specific antigen expression in all of the prostate tissues. These results indicate that PTI-1 may be a member of a class of oncogenes that could affect protein translation and contribute to carcinoma development in human prostate and other tissues. The approaches used, rapid expression cloning with the CREF-Trans 6 system and the DD strategy, should prove widely applicable for identifying and cloning additional human oncogenes.
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