ArticleThe EMBO journal1995
Experimental evidence for RNA trans-splicing in mammalian cells.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Comparative genomics reveals LINE-1 recombination with diverse RNAs.Cell genomics · 2026Article
- RNA exon editing: Splicing the way to treat human diseases.Molecular therapy. Nucleic acids · 2024Review
- Fusion Genes and RNAs in Cancer Development.Non-coding RNA · 2021Review
- Discovery and characterization of novel trans-spliced products of human polyoma JC virus late transcripts from PML patients.Journal of cellular physiology · 2018Article
- Article
- Evolutionary Insights into RNA trans-Splicing in Vertebrates.Genome biology and evolution · 2016Review
- Homologous SV40 RNA trans-splicing: a new mechanism for diversification of viral sequences and phenotypes.RNA biology · 2013Article
- Functional pre- mRNA trans-splicing of coactivator CoAA and corepressor RBM4 during stem/progenitor cell differentiation.The Journal of biological chemistry · 2009Article
- Proximity-dependent and proximity-independent trans-splicing in mammalian cells.RNA (New York, N.Y.) · 2008Article
- RNA recombination in vivo in the absence of viral replication.Journal of virology · 2004Article
- Exon repetition: a major pathway for processing mRNA of some genes is allele-specific.Nucleic acids research · 2004Article
- Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing.The Journal of clinical investigation · 2003Review
- Molecular mechanisms governing Pcdh-gamma gene expression: evidence for a multiple promoter and cis-alternative splicing model.Genes & development · 2002Article
- Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
- Article
- Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing.Gene therapy · 2000Article
- Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene.Genome research · 2000Article
- RNA molecules containing exons originating from different members of the cytochrome P450 2C gene subfamily (CYP2C) in human epidermis and liver.Nucleic acids research · 1999Article
- Exon repetition in mRNA.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present evidence that mammalian cells have the ability to generate functional mRNA molecules by trans-splicing. Rat cells, transformed by an early SV40 DNA fragment (Bst/Bam) synthesize a truncated T antigen (T1 antigen), although the cells do not have a direct sequence homology for the T1 antigen at the DNA level. The Bst/Bam DNA fragment encodes exclusively for the second SV40 T antigen exon (aa 83-708) and contains the entire small t antigen intron. To synthesize the corresponding mRNA (T1 mRNA), the cells utilize a cryptic 5' splice site within the second exon (codons for aa 131/132) as donor site and the upstream small t antigen 3' splice site as the acceptor site. Since these sites are in an inverted order on the pre-mRNA, two Bst/Bam transcripts are required to generate one T1 mRNA molecule. HeLa cell nuclear extracts also performed the trans-splicing reaction in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.