ArticleThe EMBO journal1995
Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 316 citations in OpenAlex.
- Transcriptional and post-translational regulation of aquaporins in plants.Stress biology · 2026Review
- Homeostasis of ammonium and nitrate in plants.Quantitative plant biology · 2026Review
- Post-translational modification acts as a digital like switch influencing AtPIP2;1 water and cation permeability.Scientific reports · 2025Article
- Root remodeling mechanisms and salt tolerance trade-offs: The roles of HKT1, TMAC2, and TIP2;2 in Arabidopsis.PLoS genetics · 2025Article
- Red LED Light Improves Pepper (International journal of molecular sciences · 2023Article
- Large-Scale Quantitative Proteomic Analysis during Different Stages of Somatic Embryogenesis inCurrent issues in molecular biology · 2023Article
- Exploring aquaporin functions during changes in leaf water potential.Frontiers in plant science · 2023Review
- Screening and identification of salt-tolerance genes in Sophora alopecuroides and functional verification of SaAQP.Planta · 2021Article
- Phosphorylation of TIP3 Aquaporins duringCells · 2019Article
- Sucrose-induced Receptor Kinase 1 is Modulated by an Interacting Kinase with Short Extracellular Domain.Molecular & cellular proteomics : MCP · 2019Article
- Aquaporins influence seed dormancy and germination in response to stress.Plant, cell & environment · 2019Article
- Water transport, perception, and response in plants.Journal of plant research · 2019Review
- Plant and Mammal Aquaporins: Same but Different.International journal of molecular sciences · 2018Review
- The involvement of calmodulin and protein kinases in the upstream of cytosolic and nucleic calcium signaling induced by hypoosmotic shock in tobacco cells.Plant signaling & behavior · 2018Article
- Identification of Metabolites and Transcripts Involved in Salt Stress and Recovery in Peanut.Frontiers in plant science · 2018Article
- Plant and animal aquaporins crosstalk: what can be revealed from distinct perspectives.Biophysical reviews · 2017Review
- A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti.Nature biotechnology · 2016Article
- The Arabidopsis AtPP2CA Protein Phosphatase Inhibits the GORK K+ Efflux Channel and Exerts a Dominant Suppressive Effect on Phosphomimetic-activating Mutations.The Journal of biological chemistry · 2016Article
- Tonoplast Aquaporins Facilitate Lateral Root Emergence.Plant physiology · 2016Article
- The Aquaporin Splice Variant NbXIP1;1α Is Permeable to Boric Acid and Is Phosphorylated in the N-terminal Domain.Frontiers in plant science · 2016Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vacuolar membrane protein alpha-TIP is a seed-specific protein of the Major Intrinsic Protein family. Expression of alpha-TIP in Xenopus oocytes conferred a 4- to 8-fold increase in the osmotic water permeability (Pf) of the oocyte plasma membrane, showing that alpha-TIP forms water channels and is thus a new aquaporin. alpha-TIP has three putative phosphorylation sites on the cytoplasmic side of the membrane (Ser7, Ser23 and Ser99), one of which (Ser7) has been shown to be phosphorylated. We present several lines of evidence that the activity of this aquaporin is regulated by phosphorylation. First, mutation of the putative phosphorylation sites in alpha-TIP (Ser7Ala, Ser23Ala and Ser99Ala) reduced the apparent water transport activity of alpha-TIP in oocytes, suggesting that phosphorylation of alpha-TIP occurs in the oocytes and participates in the control of water channel activity. Second, exposure of oocytes to the cAMP agonists 8-bromoadenosine 3',5'-cyclic monophosphate, forskolin and 3-isobutyl-1-methylxanthine, which stimulate endogenous protein kinase A (PKA), increased the water transport activity of alpha-TIP by 80-100% after 60 min. That the protein can be phosphorylated by PKA was demonstrated by phosphorylating alpha-TIP in isolated oocyte membranes with the bovine PKA catalytic subunit. Third, the integrity of the three sites at positions 7, 23 and 99 was necessary for the cAMP-dependent increase in the Pf of oocytes expressing alpha-TIP, as well as for in vitro phosphorylation of alpha-TIP. These findings demonstrate that the alpha-TIP water channel can be modulated via phosphorylation of Ser7, Ser23 and Ser99.(ABSTRACT TRUNCATED AT 250 WORDS)
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