ArticleJournal of virology1995
Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp120.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Emergency Services of Viral RNAs: Repair and Remodeling.Microbiology and molecular biology reviews : MMBR · 2018Review
- A replication-competent foot-and-mouth disease virus expressing a luciferase reporter.Journal of virological methods · 2017Article
- An infectious recombinant foot-and-mouth disease virus expressing a fluorescent marker protein.The Journal of general virology · 2013Article
- Evolution of poliovirus defective interfering particles expressing Gaussia luciferase.Journal of virology · 2012Article
- Establishment of infectious HCV virion-producing cells with newly designed full-genome replicon RNA.Archives of virology · 2011Article
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- Viable polioviruses that encode 2A proteins with fluorescent protein tags.Journal of virology · 2010Article
- An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys.Journal of virology · 2007Article
- Expression of a membrane-anchored glycoprotein, the influenza virus hemagglutinin, by dicistronic replicons derived from the poliovirus genome.Journal of virology · 2002Article
- Novel design architecture for genetic stability of recombinant poliovirus: the manipulation of G/C contents and their distribution patterns increases the genetic stability of inserts in a poliovirus-based RPS-Vax vector system.Journal of virology · 2002Article
- Expression of brain-derived neurotrophic factor in the central nervous system of mice using a poliovirus-based vector.Journal of neurovirology · 2002Article
- Genetic analysis of a poliovirus/hepatitis C virus chimera: new structure for domain II of the internal ribosomal entry site of hepatitis C virus.Journal of virology · 2001Article
- Characterization of recombinant hepatitis A virus genomes containing exogenous sequences at the 2A/2B junction.Journal of virology · 2001Article
- Recombinant yellow fever viruses are effective therapeutic vaccines for treatment of murine experimental solid tumors and pulmonary metastases.Journal of virology · 2000Article
- Genetic analysis of a poliovirus/hepatitis C virus (HCV) chimera: interaction between the poliovirus cloverleaf and a sequence in the HCV 5' nontranslated region results in a replication phenotype.Journal of virology · 2000Article
- Article
- Brefeldin A inhibits cell-free, de novo synthesis of poliovirus.Journal of virology · 1998Article
- Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames.Journal of virology · 1998Article
- Toward a poliovirus-based simian immunodeficiency virus vaccine: correlation between genetic stability and immunogenicity.Journal of virology · 1997Article
- Poliovirus chimeras replicating under the translational control of genetic elements of hepatitis C virus reveal unusual properties of the internal ribosomal entry site of hepatitis C virus.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
On the basis of previous studies of dicistronic (dc) polioviruses that carried two internal ribosomal entry sites (L. Alexander, H.-H. Lu, and E. Wimmer, Proc. Natl. Acad. Sci. USA 91:1406-1410, 1994; A. Molla, S. K. Jang, A. V. Paul, Q. Reuer, and E. Wimmer, Nature [London] 356:255-257, 1992), we have constructed a variety of dc polioviruses which express foreign genetic elements that were inserted either between two internal ribosomal entry site elements upstream of the poliovirus open reading frame (pPNENPO derivatives) or upstream of the open reading frame for the poliovirus proteinase 2Apro (pDI-E2A derivatives). Surprisingly, the addition of an N-terminal secretory pathway signal sequence to the open reading frame of the inserted foreign sequences (specifying either truncated versions of human immunodeficiency virus type 1 [HIV-1] gp120 or chloramphenicol acetyltransferase) resulted in a null phenotype, whereas removal of the signal sequence led to the production of viable viruses. Constructs that carried a foreign gene with a signal sequence were negative in RNA synthesis, an observation that suggested a very early block in viral replication. The insertion of transmembrane sequences downstream of the leader sequence did not reverse the replication block. Studies of dc polioviruses that encoded the truncated versions of HIV-1 gp120 showed an increase in genetic stability that correlated with a decrease in the size of the insert. A dc construct that contained a minigene encoding the principal neutralization determinant of HIV-1 produced a stable virus that retained the foreign sequence through multiple passages in cultured cells. These data indicate that dc polioviruses have potential as vaccines for the expression of small foreign epitopes.
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