Evidence map›Paper›PMID 7540630›Full record

ArticleJournal of clinical microbiology1995

Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping).

O Okwumabua, J Staats, M M Chengappa

Open access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Tools for Molecular Epidemiology ofPathogens (Basel, Switzerland) · 2020
    Review
  5. Review
  6. Article
  7. Article
  8. Detection and molecular typing of Streptococcus suis in tonsils from live pigs in France.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2007
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Genetic diversity of Streptococcus suis serotypes 2 and 1/2 isolates recovered from carrier pigs in closed herds.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2002
    Article
  14. Article
  15. Article
  16. Article
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  18. Article
  19. Article
  20. Dilemma of the virulence of Strptococcus suis strains.Journal of clinical microbiology · 1999
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

O OkwumabuaDepartment of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
J Staats
M M Chengappa
Kansas State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Whole-cell chromosomal digests of 54 isolates of Streptococcus suis encompassing all known serotypes from a geographically varied collection were examined by PstI restriction fragment length polymorphisms and then hybridized with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from a mixture of 16S and 23S rRNAs from Escherichia coli MRE600. The hybridization patterns showed genetic heterogeneity within and between S. suis serotypes. Most isolates (87%) representing 28 serotypes contained a common band at approximately 1.8 kb. However, 13% of the isolates representing seven serotypes lacked the 1.8-kb band, indicating that the species as currently defined is diverse. Nonetheless, the 1.8-kb band may be a useful genotypic marker for identification of most S. suis isolates. We tested the ability of this technique to discriminate between virulent and avirulent S. suis type 2 isolates. A virulent strain of S. suis type 2 could be distinguished from avirulent strains by the presence of specific bands. No correlation was obvious between band pattern and hemolysin production.

Indexed as

Polymorphism, Restriction Fragment LengthAnimalsDNA, BacterialDNA, ComplementaryGenetic MarkersGenetic VariationHumansRNA, BacterialRNA, RibosomalSerotypingStreptococcus suisSwineDNA, BacterialDNA, ComplementaryGenetic MarkersRNA, BacterialRNA, Ribosomal

Identifiers

PMID7540630
PMCPMC228077
OpenAlexW2171995977

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.