Trial reportJournal of clinical microbiology1995
Simplified PCR for detection of Haemophilus ducreyi and diagnosis of chancroid.
Trial report in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Polymerase chain reaction detection of Haemophilus ducreyi DNA.Sexually transmitted infections · 1998Trial
- Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans.Clinical microbiology reviews · 2014Review
- Possible reasons for an increase in the proportion of genital ulcers due to herpes simplex virus from a cohort of female bar workers in Tanzania.Sexually transmitted infections · 2007Article
- Chancroid: clinical manifestations, diagnosis, and management.Sexually transmitted infections · 2003Review
- Rapid detection of sepsis complicating acute necrotizing pancreatitis using polymerase chain reaction.World journal of gastroenterology · 2001Article
- Diagnosing genital ulcer disease in a clinic for sexually transmitted diseases in Amsterdam, The Netherlands.Journal of clinical microbiology · 2001Article
- Diagnostic tests for chancroid.Sexually transmitted infections · 2000Review
- Development of a serological test for Haemophilus ducreyi for seroprevalence studies.Journal of clinical microbiology · 2000Article
- The Use of Molecular Techniques for the Diagnosis and Epidemiologic Study of Sexually Transmitted Infections.Current infectious disease reports · 2000Article
- Asymptomatic carriage of Haemophilus ducreyi confirmed by the polymerase chain reaction.Genitourinary medicine · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simplified PCR was developed for detection of Haemophilus ducreyi in samples from chancroid patients. The strategy included a straightforward chloroform extraction sample preparation method, a one-tube nested PCR to minimize contamination risks, and a colorimetric method for detection of products. Primers were designed from published nucleotide sequences of the 16S rRNA gene of H. ducreyi, with longer outer primers for annealing at a higher temperature and shorter inner primers labelled with biotin and digoxigenin for binding with avidin and colorimetric detection. The PCR technique detected all 35 strains of H. ducreyi tested, from four different geographical regions, and was negative for other, related strains of bacteria and for the common contaminating bacteria tested. Of 25 samples from H. ducreyi culture-positive chancroid patients, 24 were PCR positive and 1 produced a weak reaction. Of 83 samples from clinical cases of chancroid in the Republic of South Africa, 69 were PCR positive. The sensitivity of PCR compared with that of clinical diagnosis was 83%. All 50 negative control samples were negative. Encouraging results were also obtained with a consecutive series of 25 genital ulcer patients in Tanzania, of whom 9 were PCR positive. The adaptations of this simplified PCR strategy, at the sensitivity and specificity levels obtained, mean it will be useful for detection of H. ducreyi in areas where the organism is endemic, particularly where testing by culture is difficult or impossible.
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