ArticleThe Journal of cell biology1995
Fibrin II induces endothelial cell capillary tube formation.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 137 citations in OpenAlex.
- Vascular leakage and angiogenesis in wound healing: a review.Molecular biology reports · 2025Review
- Application and Mechanism of Adipose Tissue-Derived Microvascular Fragments in Tissue Repair and Regeneration.Biomolecules · 2025Review
- Construction of biomimetic cell-sheet-engineered periosteum with a double cell sheet to repair calvarial defects of rats.Journal of orthopaedic translation · 2023Article
- Current View on the Molecular Mechanisms Underlying Fibrin(ogen)-Dependent Inflammation.Thrombosis and haemostasis · 2022Review
- Fibrin polymer on the surface of biomaterial implants drives the foreign body reaction.Biomaterials · 2021Article
- Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice.Haematologica · 2019Article
- Engineering biofunctional in vitro vessel models using a multilayer bioprinting technique.Scientific reports · 2018Article
- In Vitro Multitissue Interface Model Supports Rapid Vasculogenesis and Mechanistic Study of Vascularization across Tissue Compartments.ACS applied materials & interfaces · 2016Article
- Endothelial matrix assembly during capillary morphogenesis: insights from chimeric TagRFP-fibronectin matrix.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Engineering vascularized bone grafts by integrating a biomimetic periosteum and β-TCP scaffold.ACS applied materials & interfaces · 2014Article
- Dual delivery of vascular endothelial growth factor and hepatocyte growth factor coacervate displays strong angiogenic effects.Macromolecular bioscience · 2014Article
- Sculpting the blank slate: how fibrin's support of vascularization can inspire biomaterial design.Acta biomaterialia · 2014Review
- Different angiogenic abilities of self-setting calcium phosphate cement scaffolds consisting of different proportions of fibrin glue.BioMed research international · 2014Article
- Influence of levamisole and other angiogenesis inhibitors on angiogenesis and endothelial cell morphology in vitro.Cancers · 2013Article
- In vitro construction of scaffold-free bilayered tissue-engineered skin containing capillary networks.BioMed research international · 2013Article
- Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen.Protein & cell · 2012Article
- Vascular hyperpermeability, angiogenesis, and stroma generation.Cold Spring Harbor perspectives in medicine · 2012Review
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- Preparation of chitosan films using different neutralizing solutions to improve endothelial cell compatibility.Journal of materials science. Materials in medicine · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We studied the formation of capillary tubes by endothelial cells which were sandwiched between two fibrin gels under serum-free conditions. After formation of the overlying fibrin gel, the endothelial cell monolayer rearranged into an extensive net of capillary tubes. Tube formation was apparent at 5 h and was fully developed by 24 h. The capillary tubes were vacuolated, and both intracellular and intercellular lumina were present. Maximal tube formation was observed with fibrin II (which lacks both fibrinopeptide A and B), minimal tube formation with fibrin I (which lacks only fibrinopeptide A), and complete absence of tube formation with fibrin 325 (which lacks the NH2-terminal beta 15-42 sequence, in addition to fibrinopeptides A and B). The inability of fibrin 325 to stimulate capillary tube formation supports the idea that beta 15-42 plays an important role in this process, and its importance was confirmed by the finding that exogenous soluble beta 15-42 inhibited fibrin II-induced capillary tube formation. This effect was specific for fibrin, since beta 15-42 did not inhibit tube formation by endothelial cells sandwiched between collagen gels. The interaction of the apical surface of the endothelial cell with the overlying fibrin II gel, as opposed to the underlying fibrin gel upon which the cells were seeded, was necessary for capillary tube formation. These studies suggest that the beta 15-42 sequence of fibrin interacts with a component of the apical cell surface and that this interaction plays a fundamental role in the induction of endothelial capillary tube formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.