ArticleBiophysical journal1995
Cell volume measured by total internal reflection microfluorimetry: application to water and solute transport in cells transfected with water channel homologs.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Determination of cell volume as part of metabolomics experiments.American journal of physiology. Cell physiology · 2021Article
- Single-file transport of water through membrane channels.Faraday discussions · 2018Article
- Rapid Identification of Novel Inhibitors of the Human Aquaporin-1 Water Channel.Chemical biology & drug design · 2016Article
- Article
- Aquaporins: important but elusive drug targets.Nature reviews. Drug discovery · 2014Review
- Wnt signalling pathway parameters for mammalian cells.PloS one · 2012Article
- Modelling the swelling assay for aquaporin expression.Journal of mathematical biology · 2008Article
- Contribution of aquaporins to cellular water transport observed by a microfluidic cell volume sensor.Analytical chemistry · 2008Article
- Functional involvement of Annexin-2 in cAMP induced AQP2 trafficking.Pflugers Archiv : European journal of physiology · 2008Article
- From membrane pores to aquaporins: 50 years measuring water fluxes.Journal of biological physics · 2007Article
- Comparative functional analysis of aquaporins/glyceroporins in mammals and anurans.Mammalian genome : official journal of the International Mammalian Genome Society · 2007Review
- Effect of dDAVP on basolateral cell surface water permeability in the outer medullary collecting duct.European biophysics journal : EBJ · 2003Article
- Cell volume kinetics of adherent epithelial cells measured by laser scanning reflection microscopy: determination of water permeability changes of renal principal cells.Biophysical journal · 2001Article
- Osmotic water permeabilities of cultured, well-differentiated normal and cystic fibrosis airway epithelia.The Journal of clinical investigation · 2000Article
- Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum.Biophysical journal · 1999Article
- Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
- High intracellular chloride delays the activation of the volume-sensitive chloride conductance in mouse L-fibroblasts.The Journal of physiology · 1999Article
- Expression of aquaporin-4 in fast-twitch fibers of mammalian skeletal muscle.The Journal of clinical investigation · 1998Article
- Defective aquaporin-2 trafficking in nephrogenic diabetes insipidus and correction by chemical chaperones.The Journal of clinical investigation · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Total internal reflection (TIR) microfluorimetry was established as a method to measure continuously the volume of adherent cells and applied to measure membrane permeabilities in cells transfected with water channel homologs. Cytosol was labeled with the membrane-impermeant fluorophore calcein. Fluorescence was excited by the TIR evanescent field in a thin section of cytosol (approximately 150 nm) adjacent to the cell-substrate interface. Because cytosolic fluorophore number per cell remains constant, the TIR fluorescence signal should be inversely related to cell volume. For small volume changes in Sf-9 and LLC-PK1 cells, relative TIR fluorescence was nearly equal to inverse relative cell volume; deviations from the ideal were modeled theoretically. To measure plasma membrane osmotic water permeability, Pf, the time course of osmotically induced cell volume change was inferred from the TIR fluorescence signal. LLC-PK1 cells expressing the CHIP28 water channel had an HgCl2-sensitive, threefold increase in Pf compared to nontransfected cells (Pf = 0.0043 cm/s at 10 degrees C). Solute permeability was measured from the TIR fluorescence time course in response to solute gradients. Glycerol permeability in Sf-9 cells expressing the water channel homolog GLIP was (1.3 +/- 0.2) x 10(-5) cm/s (22 degrees C), greater than that of (0.36 +/- 0.04) x 10(-5) cm/s (n = 4, p < 0.05) for control cells, indicating functional expression of GLIP. Water and urea permeabilities were similar in GLIP-expressing and control cells. The TIR method should be applicable to the study of water and solute permeabilities and cell volume regulation in cells of arbitrary shape and size.
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