ReviewTrends in genetics : TIG1995
Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis.
Review in Trends in genetics : TIG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 174 citations in OpenAlex.
- Rapid, biochemical tagging of cellular activity history in vivo.Nature methods · 2024Article
- A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning.Nature communications · 2024Article
- Rapid, biochemical tagging of cellular activity history in vivo.bioRxiv : the preprint server for biology · 2024Article
- Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells.Journal of biological engineering · 2023Article
- Synthetic polycistronic sequences in eukaryotes.Synthetic and systems biotechnology · 2021Review
- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components.The Journal of biological chemistry · 2020Article
- Construction of a highly efficient display system for baculovirus and its application on multigene co-display.Molecular genetics and genomics : MGG · 2018Article
- Article
- Reprogramming eukaryotic translation with ligand-responsive synthetic RNA switches.Nature methods · 2016Article
- Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Comparison of internal ribosome entry site (IRES) and Furin-2A (F2A) for monoclonal antibody expression level and quality in CHO cells.PloS one · 2013Article
- An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells.PloS one · 2013Article
- Bicistronic DNA vaccines simultaneously encoding HIV, HSV and HPV antigens promote CD8⁺ T cell responses and protective immunity.PloS one · 2013Article
- Conditional gene expression in the mouse inner ear using Cre-loxP.Journal of the Association for Research in Otolaryngology : JARO · 2012Review
- Article
- Article
- Transcripts expressed using a bicistronic vector pIREShyg2 are sensitized to nonsense-mediated mRNA decay.BMC molecular biology · 2010Article
- Bipartite vectors for co-expression of a growth factor cDNA and short hairpin RNA against an apoptotic gene.The journal of gene medicine · 2009Article
- Sympathetic nervous system-targeted neuropeptide Y overexpression in mice enhances neointimal formation in response to vascular injury.Peptides · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modification of the genetic content of cultured cells or of whole animals is now a key strategy in both basic biological research and applied biotechnology. Yet obtaining the desired level and specificity of expression of an introduced gene remains highly problematic. One solution could be to couple expression of a transgene to that of an appropriate intact genomic locus. The identification and functional characterization of RNA sequences known as internal ribosome entry sites now offer the possibility of achieving precise control of transgene expression through the generation of dicistronic fusion mRNAs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.