ArticleThe Journal of physiology1995
Conductance and kinetics of single cGMP-activated channels in salamander rod outer segments.
Article in The Journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Potentiation of the GABABiophysical journal · 2024Article
- The structure of the native CNGA1/CNGB1 CNG channel from bovine retinal rods.Nature structural & molecular biology · 2022Article
- Cyclic nucleotide-gated channel block by hydrolysis-resistant tetracaine derivatives.Journal of medicinal chemistry · 2011Article
- Gating of cyclic nucleotide-gated (CNGA1) channels by cGMP jumps and depolarizing voltage steps.Biophysical journal · 2006Article
- Allosteric effects of external K+ ions mediated by the aspartate of the GYGD signature sequence in the Kv2.1 K+ channel.Pflugers Archiv : European journal of physiology · 2006Article
- K channel subconductance levels result from heteromeric pore conformations.The Journal of general physiology · 2005Article
- Effects of permeating ions and cGMP on gating and conductance of rod-type cyclic nucleotide-gated (CNGA1) channels.The Journal of physiology · 2004Article
- Influence of permeant ions on gating in cyclic nucleotide-gated channels.The Journal of general physiology · 2003Article
- Subunit modification and association in VR1 ion channels.BMC neuroscience · 2002Article
- Hidden Markov model analysis of intermediate gating steps associated with the pore gate of shaker potassium channels.The Journal of general physiology · 2001Article
- Cyclic nucleotide-gated channels: shedding light on the opening of a channel pore.Nature reviews. Neuroscience · 2001Review
- Effects of ultraviolet modification on the gating energetics of cyclic nucleotide-gated channels.The Journal of general physiology · 2000Article
- The kinetic and physical basis of K(ATP) channel gating: toward a unified molecular understanding.Biophysical journal · 2000Article
- Coexpression of alpha and beta subunits of the rod cyclic GMP-gated channel restores native sensitivity to cyclic AMP: role of D604/N1201.Biophysical journal · 2000Article
- Gating by cyclic GMP and voltage in the alpha subunit of the cyclic GMP-gated channel from rod photoreceptors.The Journal of general physiology · 1999Article
- Opening mechanism of a cyclic nucleotide-gated channel based on analysis of single channels locked in each liganded state.The Journal of general physiology · 1999Article
- Divalent cation selectivity is a function of gating in native and recombinant cyclic nucleotide-gated ion channels from retinal photoreceptors.The Journal of general physiology · 1999Article
- Membrane current noise in dark-adapted and light-adapted isolated retinal rods of the larval tiger salamander.The Journal of physiology · 1998Article
- Cyclic GMP-gated channels in a sympathetic neuron cell line.The Journal of general physiology · 1997Article
- Single-channel properties of ionic channels gated by cyclic nucleotides.Biophysical journal · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. The conductance and kinetics of single 3',5'-cyclic guanosine monophosphate (cGMP)-activated channels of retinal rod outer segments were studied in inside-out membrane patches. The size of the single channel currents was increased by using low concentrations of divalent cations. 2. At saturating cGMP concentration, the current flickered at high frequency. Occasionally, the current was interrupted by closures lasting tens or hundreds of milliseconds. At +50 mV the maximum current during an opening was slightly more than 1 pA, but the open channel level was poorly resolved due to the speed of the gating transitions. 3. Amplitude histograms confirmed the presence of a sublevel of current, roughly a quarter the size of the peak current, at low cGMP concentrations. The fraction of time in the sublevel decreased with increasing cGMP concentration, suggesting that the sublevel may be due to opening by the partially liganded channel. 4. Consistent with previous macroscopic current recordings, single channel activation by cGMP had an apparent dissociation constant of 8.6 microM, and a Hill coefficient of 2.8. 5. At saturating cGMP concentrations, the channel was modelled as a two-state system with the following parameters. The open channel conductance was 25 pS. The opening rate constant, beta, was 1.5 x 10(4) s-1 at 0 mV, and had a voltage sensitivity equivalent to the movement of 0.23 electronic charges outward through the membrane electric field. The closing rate constant, alpha, was 2.1 x 10(4) s-1 and was voltage insensitive. Assuming that the open-state chord conductance was voltage independent, the inferred voltage dependence of beta largely accounted for the outward rectification in the steady-state macroscopic current-voltage relation of multichannel patches, at saturating cGMP concentration.
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