ArticleNucleic acids research1995
RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.).
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme.Nature communications · 2026Article
- Molecular phylogenetic relationships based on chloroplast genomes of Zingiberaceae species: Insights into evolution and adaptation to extreme environments.Frontiers in plant science · 2025Article
- Comparative plastome analysis of Arundinelleae (Poaceae, Panicoideae), with implications for phylogenetic relationships and plastome evolution.BMC genomics · 2024Article
- Chloroplast genome structure analysis ofFrontiers in plant science · 2024Article
- Insights into adaptive evolution of plastomes in Stipa L. (Poaceae).BMC plant biology · 2022Article
- Review
- Structural Plastome Evolution in Holoparasitic Hydnoraceae with Special Focus on Inverted and Direct Repeats.Genome biology and evolution · 2022Article
- Analysis of six chloroplast genomes provides insight into the evolution of Chrysosplenium (Saxifragaceae).BMC genomics · 2020Article
- Comprehensive genomic analyses with 115 plastomes from algae to seed plants: structure, gene contents, GC contents, and introns.Genes & genomics · 2020Article
- Unraveling the role of the enigmatic MatK maturase in chloroplast group IIA intron excision.Plant direct · 2020Article
- Plastome of mycoheterotrophicPeerJ · 2019Article
- Characterization of the Chloroplast Genome Sequence ofMolecules (Basel, Switzerland) · 2018Article
- Ectopic Transplastomic Expression of a Synthetic MatK Gene Leads to Cotyledon-Specific Leaf Variegation.Frontiers in plant science · 2018Article
- A purification strategy for analysis of the DNA/RNA-associated sub-proteome from chloroplasts of mustard cotyledons.Frontiers in plant science · 2014Article
- Multiple checkpoints for the expression of the chloroplast-encoded splicing factor MatK.Plant physiology · 2013Article
- Mechanisms of functional and physical genome reduction in photosynthetic and nonphotosynthetic parasitic plants of the broomrape family.The Plant cell · 2013Article
- The evolution of chloroplast genes and genomes in ferns.Plant molecular biology · 2011Article
- The evolution of the plastid chromosome in land plants: gene content, gene order, gene function.Plant molecular biology · 2011Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chloroplast trnK gene for tRNALys(UUU) from mustard contains a 2574 bp group II intron with a long open reading frame for 524 amino acids. The encoded polypeptide appears to be structurally related to mitochondrial maturases which are involved in splicing. To study the properties of the intron encoded protein, we overexpressed the trnK ORF as a beta-galactosidase fusion protein in E. coli and carried out RNA-protein binding experiments with crude bacterial extracts and the purified fusion protein. Both gel-shift and UV-crosslinking experiments revealed preferential binding to the trnK precursor transcript. Of two other RNA probes containing chloroplast group II introns, the trnG precursor was recognized by the trnK ORF protein, but the rps16 precursor was not. Competition binding experiments indicate that G-residues seem to play a role in RNA-protein interaction. RNA-binding activity of the trnK intron encoded polypeptide is consistent with its suggested function as a plastid maturase, hence justifying the assignment matK for this gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.