ArticleThe Journal of cell biology1995
Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 161 papers.
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161 citing papers in PubMed.
- Non-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections.The EMBO journal · 2026Article
- Genome folding and nuclear speckles converge to orchestrate fibroblast activation.Science advances · 2026Article
- A single cluster of RNA Polymerase II molecules is stably associated with active genes.Nature communications · 2026Article
- A single cluster of RNA Polymerase II molecules is stably associated with active genes.bioRxiv : the preprint server for biology · 2025Article
- Chromatin profiling data indicate regulatory mechanisms for differentiation during development in the acoel Hofstenia miamia.Development (Cambridge, England) · 2025Article
- ZNF32 histidine 179 and 183 single-site and double-site mutations promote nuclear speckle formation but differentially regulate the proliferation of breast cancer cells.Frontiers in cell and developmental biology · 2025Article
- RNA-mediated demixing transition of low-density condensates.Nature communications · 2023Article
- Nuclear compartmentalization as a mechanism of quantitative control of gene expression.Nature reviews. Molecular cell biology · 2021Review
- Cold shock induces novel nuclear bodies in Xenopus oocytes.Experimental cell research · 2021Review
- Targeting the Transcriptome Through Globally Acting Components.Frontiers in genetics · 2021Review
- Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.Emerging topics in life sciences · 2020Article
- Article
- Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis.Development (Cambridge, England) · 2019Article
- Organization and regulation of gene transcription.Nature · 2019Review
- Elongin B is a binding partner of the male germ cell nuclear speckle protein sperm-associated antigen 16S (SPAG16S) and is regulated post-transcriptionally in the testis.Reproduction, fertility, and development · 2019Article
- Knockdown of Heat Shock Proteins HSPA6 (Hsp70B') and HSPA1A (Hsp70-1) Sensitizes Differentiated Human Neuronal Cells to Cellular Stress.Neurochemical research · 2018Article
- A novel role forDevelopment (Cambridge, England) · 2018Article
- Differential Targeting of Hsp70 Heat Shock Proteins HSPA6 and HSPA1A with Components of a Protein Disaggregation/Refolding Machine in Differentiated Human Neuronal Cells following Thermal Stress.Frontiers in neuroscience · 2017Article
- Persistent nuclear actin filaments inhibit transcription by RNA polymerase II.Journal of cell science · 2016Article
- Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination.Nucleic acids research · 2016Article
101 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
A subpopulation of the largest subunit of RNA polymerase II (Pol II LS) is located in 20-50 discrete subnuclear domains that are closely linked to speckle domains, which store splicing proteins. The speckle-associated fraction of Pol II LS is hyperphosphorylated on the COOH-terminal domain (CTD), and it is highly resistant to extraction by detergents. A diffuse nucleoplasmic fraction of Pol II LS is relatively hypophosphorylated on the CTD, and it is easily extracted by detergents. In transcriptionally active nuclei, speckle bound hyperphosphorylated Pol II LS molecules are distributed in irregularly shaped speckle domains, which appear to be interconnected via a reticular network. When transcription is inhibited, hyperphosphorylated Pol II LS and splicing protein SC35 accumulate in speckle domains, which are transformed into enlarged, dot-like structures lacking interconnections. When cells are released from transcriptional inhibition, Pol IIO and SC35 redistribute back to the interconnected speckle pattern of transcriptionally active cells. The redistribution of Pol II and SC35 is synchronous, reversible, and temperature dependent. It is concluded that: (a) hyperphosphorylation of Pol II LS's CTD is a better indicator of its tight association to discrete subnuclear domains than its transcriptional activity; (b) during states of transcriptional inhibition, hyperphosphorylated Pol II LS can be stored in enlarged speckle domains, which under the light microscope appear to coincide with the storage sites for splicing proteins; and (c) Pol II and splicing proteins redistribute simultaneously according to the overall transcriptional activity of the nucleus.
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