ArticleJournal of bacteriology1995
Bacterial luciferase as a reporter of circadian gene expression in cyanobacteria.
Article in Journal of bacteriology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 83 citations in OpenAlex.
- Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.Nature biotechnology · 2026Article
- Clocking out and letting go to unleash green biotech applications in a photosynthetic host.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Inter-Species Redox Coupling by Flavin Reductases and FMN-Dependent Two-Component Monooxygenases Undertaking Nucleophilic Baeyer-Villiger Biooxygenations.Microorganisms · 2022Article
- Entrainment of the Circadian Clock of the Enteric Bacterium Klebsiella aerogenes by Temperature Cycles.iScience · 2019Article
- The cyanobacterial clock and metabolism.Current opinion in microbiology · 2014Review
- Active output state of the Synechococcus Kai circadian oscillator.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- An allele of the crm gene blocks cyanobacterial circadian rhythms.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Sequence determinants of circadian gene expression phase in cyanobacteria.Journal of bacteriology · 2013Article
- Article
- The itty-bitty time machine genetics of the cyanobacterial circadian clock.Advances in genetics · 2011Review
- Oscillations in supercoiling drive circadian gene expression in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Two lysine residues in the bacterial luciferase mobile loop stabilize reaction intermediates.The Journal of biological chemistry · 2009Article
- Article
- Fre Is the Major Flavin Reductase Supporting Bioluminescence from Vibrio harveyi Luciferase in Escherichia coli.The Journal of biological chemistry · 2009Article
- Choreography of the transcriptome, photophysiology, and cell cycle of a minimal photoautotroph, prochlorococcus.PloS one · 2009Article
- Regulation of circadian clock gene expression by phosphorylation states of KaiC in cyanobacteria.Journal of bacteriology · 2008Article
- Development of bioluminescent Salmonella strains for use in food safety.BMC microbiology · 2008Article
- Integrating the circadian oscillator into the life of the cyanobacterial cell.Cold Spring Harbor symposia on quantitative biology · 2007Review
- Phosphorylation of the D1 photosystem II reaction center protein is controlled by an endogenous circadian rhythm.Plant physiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To allow continuous monitoring of the circadian clock in cyanobacteria, we previously created a reporter strain (AMC149) of Synechococcus sp. strain PCC 7942 in which the promoter of the psbAI gene was fused to Vibrio harveyi luciferase structural genes (luxAB) and integrated into the chromosome. Northern (RNA) hybridization and immunoblot analyses were performed to examine changes in abundance of the luxAB mRNA, the native psbAI mRNA, and the luciferase protein to determine whether bioluminescence is an accurate reporter of psbAI promoter activity in AMC149. Under constant light conditions, the mRNA abundances of both luxAB and psbAI oscillated with a period of approximately 24 h for at least 2 days. The expression of these two genes following the same pattern: both mRNAs peaked in the subjective morning, and their troughs occurred near the end of the subjective night. The amount of luciferase protein also oscillated with a period of approximately 24 h, and the protein rhythm is in phase with the bioluminescence rhythm. The rhythm of the luciferase mRNA phase-leads the rhythms of luciferase protein and in vivo bioluminescence by several hours. Comparable results were obtained with a short-period mutant of AMC149. Together, these results indicate that the bioluminescence rhythm in AMC149 is due primarily to circadian oscillation of psbAI promoter activity in this cyanobacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.